VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743 amino acid (aa) extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity.
Mouse VEGFR2/KDR/Flk-1 Antibody (3319C)
R&D Systems | Catalog # MAB11749
Recombinant Monoclonal Antibody.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Multiplex Immunofluorescence, Immunohistochemistry, COMET
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3319C
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Product Specifications
Immunogen
Synthetic Peptide
Accession # P35918
Accession # P35918
Specificity
Detects a synthetic peptide specific for mouse VEGFR2 around amino acid 1300 in Direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mouse VEGFR2/KDR/Flk-1 Antibody (3319C)
Detection of VEGFR2/KDR/Flk-1 in Mouse Spleen via seqIF™ staining on COMET™
VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse VEGFR2/KDR/Flk-1, Monoclonal Antibody (Catalog #MAB11749) at 10ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the endothelial cells. Protocol available in COMET™ Panel Builder.
Detection of VEGFR2/KDR/Flk-1 in Mouse Liver.
VEGFR2/KDR/Flk-1 was detected in perfusion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB11749) at 0.5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of VEGFR2/KDR/Flk-1 in Mouse Kidney.
VEGFR2/KDR/Flk-1 was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Rabbit Anti-Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB11749) at 0.5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Mouse VEGFR2/KDR/Flk-1 Antibody (3319C)
Application
Recommended Usage
COMET
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
0.5-10 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse liver and mouse kidney
Sample: Perfusion fixed paraffin-embedded sections of mouse liver and mouse kidney
Multiplex Immunofluorescence
10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen
Sample: Immersion fixed paraffin-embedded sections of mouse spleen
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: VEGFR2/KDR/Flk-1
References
- Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
- Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Alternate Names
CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2
Gene Symbol
KDR
UniProt
Additional VEGFR2/KDR/Flk-1 Products
Product Documents for Mouse VEGFR2/KDR/Flk-1 Antibody (3319C)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse VEGFR2/KDR/Flk-1 Antibody (3319C)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
