Mouse VEGFR2/KDR/Flk-1 Antibody

Catalog #: MAB11749 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11749-100
MAB11749-SP

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Detection of VEGFR2/KDR/Flk-1 in Mouse Spleen via seqIF™ staining on COMET™

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Mouse VEGFR2/KDR/Flk-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects a synthetic peptide specific for mouse VEGFR2 around amino acid 1300 in Direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 3319C

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # P35918

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

0.5-10 µg/mL

Perfusion fixed paraffin-embedded sections of mouse liver and mouse kidney

Multiplex Immunofluorescence

10 µg/mL

Immersion fixed paraffin-embedded sections of mouse spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Multiplex Immunofluorescence

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Detection of VEGFR2/KDR/Flk-1 in Mouse Spleen via seqIF™ staining on COMET™ VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse VEGFR2/KDR/Flk-1, Monoclonal Antibody (Catalog #MAB11749) at 10ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the endothelial cells. Protocol available in COMET™ Panel Builder.

Immunohistochemistry

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Detection of VEGFR2/KDR/Flk-1 in Mouse Liver. VEGFR2/KDR/Flk-1 was detected in perfusion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB11749) at 0.5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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Detection of VEGFR2/KDR/Flk-1 in Mouse Kidney. VEGFR2/KDR/Flk-1 was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Rabbit Anti-Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB11749) at 0.5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743 amino acid (aa) extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity.

References

Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Entrez Gene IDs

3791 (Human); 16542 (Mouse)

Alternate Names

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2