MTCH2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94185

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-175 of human MTCH2 (NP_055157.1). VGYEPLPPTIGRNIFGRQVCQLPGLFSYAQHIASIDGRRGLFTGLTPRLCSGVLGTVVHGKVLQHYQESDKGEELGPGNVQKEVSSSFDHVIKETTREMIARSAATLITHPFHVITLRSMVQFIGRESKYCGLCDSIITIYREEGI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MTCH2 Antibody - Azide and BSA Free

MTCH2 Antibody - Azide and BSA Free

Western Blot: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] -

Western Blot: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] - Western blot analysis of lysates from wild type(WT) and MTCH2 knockout (KO) HeLa(KO) cells, using [KO Validated] MTCH2 Rabbit pAb (A12934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
MTCH2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] -

Immunocytochemistry/ Immunofluorescence: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] MTCH2 Rabbit pAb (A12934) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
MTCH2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] -

Immunocytochemistry/ Immunofluorescence: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] MTCH2 Rabbit pAb (A12934) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for MTCH2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MTCH2

The substrate transported is not yet known. Induces mitochondrial depolarization

Alternate Names

2310034D24Rik, Met-induced mitochondrial protein, MIMP, mitochondrial carrier 2, mitochondrial carrier homolog 2, mitochondrial carrier homolog 2 (C. elegans)

Gene Symbol

MTCH2

Additional MTCH2 Products

Product Documents for MTCH2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MTCH2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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