NCKAP1 Antibody

Novus Biologicals | Catalog # NBP2-26086

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-TPREYLTSHLEIR, from the internal region of the protein sequence according to NP_038464.1; NP_995314.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_038464.1; NP_995314.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.

Scientific Data Images for NCKAP1 Antibody

NCKAP1 Antibody

Flow Cytometry: NCKAP1 Antibody [NBP2-26086] -

Flow Cytometry: NCKAP1 Antibody [NBP2-26086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
NCKAP1 Antibody

Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP2-26086] -

Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP2-26086] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NCKAP1 Antibody

Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP2-26086] -

Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP2-26086] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for NCKAP1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx 100kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5ug/ml antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calculated MWt is 129kDa according to NP_038464.1 and approx. 110-125kDa bands have been observed by other sources.

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCKAP1

Is part of lamellipodial complex that controls Rac-dependent actin remodeling

Alternate Names

FLJ11291, HEM2p125Nap1, KIAA0587, Membrane-associated protein HEM-2, MGC8981, NAP 1, NAP125, NAP1Nap1, NCK-associated protein 1

Entrez Gene IDs

10787 (Human); 50884 (Mouse); 58823 (Rat)

Gene Symbol

NCKAP1

Additional NCKAP1 Products

Product Documents for NCKAP1 Antibody

Certificate of Analysis

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Product Specific Notices for NCKAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NCKAP1 Antibody

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Protocols

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