NFATC1/NFAT2 Antibody (24GB380)

Novus Biologicals | Catalog # NBP3-50269

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB380
View all NFATC1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human NFATC1/NFAT2 (Uniprot: O95644)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFATC1/NFAT2 Antibody (24GB380)

NFATC1/NFAT2 Antibody (24GB380)

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (24GB380)[NBP3-50269] -

Immunocytochemical staining of HeLa cells with NFATC1/NFAT2 antibody (1:1,000). Nuclei were stained blue with DAPI; NFATC1/NFAT2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
NFATC1/NFAT2 Antibody (24GB380)

Flow Cytometry: NFATC1/NFAT2 Antibody (24GB380)[NBP3-50269] -

Flow cytometric analysis of NFATC1/NFAT2expression in HeLa cells using NFATC1/NFAT2 antibody (1:2,000). Green, isotype control; red, NFATC1.
NFATC1/NFAT2 Antibody (24GB380)

Western Blot: NFATC1/NFAT2 Antibody (24GB380)[NBP3-50269] -

Western blotting analysis using NFATC1/NFAT2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for NFATC1/NFAT2 Antibody (24GB380)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFATC1

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Gene Symbol

NFATC1

Additional NFATC1 Products

Product Documents for NFATC1/NFAT2 Antibody (24GB380)

Certificate of Analysis

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Product Specific Notices for NFATC1/NFAT2 Antibody (24GB380)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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