NKX3.1 Antibody (0361) - BSA Free

Novus Biologicals | Catalog # NB100-1828

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 0361

Format

BSA Free
View all NKX3.1 Primary Antibodies »

Product Specifications

Immunogen

Full-length human Nkx3.1 produced in bacteria as a hexahistidine-tagged protein. [UniProt# Q99801]

Reactivity Notes

Predicted to work in human based on 100% sequence homology. Human reactivity reported in scientific literature (PMID: 22465996).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse NKX3.1 Antibody (0361) - BSA Free (NB100-1828) is a monoclonal antibody validated for use in IHC, WB and IP. Anti-NKX3.1 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NKX3.1 Antibody (0361) - BSA Free

Western Blot: NKX3.1 Antibody (0361)BSA Free [NB100-1828]

Western Blot: NKX3.1 Antibody (0361)BSA Free [NB100-1828]

Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - Analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) - BSA Free [NB100-1828]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) - BSA Free [NB100-1828]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - Staining of human chondrosarcoma tissue with NKX3.1 monoclonal antibody. IHC-P image submitted by a verified customer review.
Western Blot: NKX3.1 Antibody (0361)BSA Free [NB100-1828]

Western Blot: NKX3.1 Antibody (0361)BSA Free [NB100-1828]

Western Blot: Nkx3.1 Antibody (0361) [NB100-1828] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute.
Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) - BSA Free [NB100-1828]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) - BSA Free [NB100-1828]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - NKX3.1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Mouse Anti-Mouse NKX3.1 (0361) Monoclonal Antibody (Catalog # NB100-1828) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei in sperm cells.
Knockdown Validated: NKX3.1 Antibody (0361) - BSA Free [NB100-1828]

Knockdown Validated: NKX3.1 Antibody (0361) - BSA Free [NB100-1828]

NKX3-1-Antibody-0361-Knockdown-Validated-NB100-1828-img0009.jpg
NKX3.1 Antibody (0361) - BSA Free

Western Blot: NKX3.1 Antibody (0361) - BSA Free [NB100-1828] -

Western Blot: NKX3.1 Antibody (0361) - BSA Free [NB100-1828] - SALL2 transcriptionally upregulates ESR1 in breast cancerARNA‐seq analysis of ESR1 mRNA levels in 9 paired pre‐tamoxifen‐treated primary breast cancer tissues & relapsed tamoxifen‐resistant breast cancer tissues.BqRT–PCR analysis of ESR1 expression in 9 paired pre‐tamoxifen‐treated primary breast cancer tissues & relapsed tamoxifen‐resistant breast cancer tissues. GAPDH was used as an internal control.C, DWB analysis of SALL2 (A) & NKX3‐1 (B) expression in the indicated cells transfected with Ri‐Vector (V‐Ri) or shRNAs (Ri#1/2) against SALL2 or NKX3‐1. alpha ‐Tubulin was used as the loading control.E, FqRT–PCR analysis of ESR1 expression in the indicated cells transfected with Ri‐Vector or shRNAs (Ri#1/2) against SALL2 or NKX3‐1.Data information: In (A), P‐values were determined by two‐tailed paired Student's t‐test. In (B), data are presented as mean ± SD, & P‐values were determined by two‐tailed unpaired Student's t‐test, n = 3. In (E & F), data are presented as mean ± SD, & P‐values were determined by one‐way ANOVA test, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., no significance. Exact P‐values are specified in Appendix Table S10.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31657150), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for NKX3.1 Antibody (0361) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Western Blot

2 ug/ml
Application Notes
This Nkx3.1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.

Reviewed Applications

Read 3 reviews rated 4.3 using NB100-1828 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX3.1

NKX3.1 (homeobox protein Nkx-3.1) is a homeodomain nuclear transcription factor expressed predominately in the prostate with very low testicular expression and is highly important in prostate biology. NKX3.1 interacts with SRF, SPDEF, WDR77 etc. and TOPORS polyubiquitinates NKX3-1 for inducing its proteasomal degradation regulation. NKX3.1 binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and act as a transcriptional repressor for playing important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Additionally, NKX3.1 functions as a prostate-specific tumor suppressor and diminished NKX3.1 expression is a consistent feature in human/mouse prostate cancers, wherein its expression is lost early in tumorigenesis suggesting that NKX3.1 loss is an early step in the progression to malignant disease.

Long Name

NK3 Homeobox 1

Alternate Names

BAPX2, NKX3-1, NKX3A

Gene Symbol

NKX3-1

Additional NKX3.1 Products

Product Documents for NKX3.1 Antibody (0361) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NKX3.1 Antibody (0361) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NKX3.1 Antibody (0361) - BSA Free

Customer Reviews for NKX3.1 Antibody (0361) - BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
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Customer Images

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  • NKX3.1 Antibody (0361)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Chondrosarcoma
    Species: Human
    Verified Customer | Posted 08/04/2021
    NKX3.1 monoclonal antibody in chondrosarcoma tissue
    NKX3.1 Antibody (0361) - BSA Free NB100-1828
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Prostate tissue
    Species: Mouse
    Verified Customer | Posted 04/19/2016
  • Name: Ronald Miller
    Application: Western Blot
    Sample Tested: Cell lysate from HeLa cells
    Species: Human
    Verified Customer | Posted 11/20/2014
    Western blot for NKX3.1 in HeLa cells
    NKX3.1 Antibody (0361) - BSA Free NB100-1828

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Protocols

View specific protocols for NKX3.1 Antibody (0361) - BSA Free (NB100-1828):

NKX3.1 Antibody (0361):
Western Blot Protocol

1. Perform SDS-PAGE (4-12%) on samples to be analyzed, loading 40 ug of total protein per lane.

2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.

3. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.

4. Rinse the blot in TBS for approximately 5 minutes.

5. Block the membrane using 5% non-fat dry milk + 1% BSA in TBS for 2 hours at room temperature.

6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.

7. Dilute the mouse anti-Nkx3.1 primary antibody (NB 100-1828) in blocking buffer and incubate 1 hour at room temperature.

8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.

9. Apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.

10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).

11. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Pierce's ECL).

Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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FAQs for NKX3.1 Antibody (0361) - BSA Free

Showing  1 - 2 的 2 FAQs Showing All

    • Q: I have three kinds of anti-mouse 2nd Ab; HRP-conjugated anti-mouse Ig G, Ig G2, Ig G2A. Are these all proper for NB100-1828 in WB or IHC?
      A: For western blot we have used a Mouse IgG secondary antibody NB7539 . This type of secondary will also be suitable for both western blot and IHC.

    • Q: Today we received an inquiry from our customer who was interested in your Nkx3.1 Antibody, #NB100-1828. The customer has a plan to use this antibody in immunohistochemical staining with her paraffin section samples from mouse. The customer would like to have IHC images obtained with #NB100-1828. In addition, the customer would like to know the detailed protocol (antibody concentration, antigen retrieval method, buffer condition and so on) which was used when checking the IHC Application.
      A: Since this is a product we have had around for some time, I was unable to find any data on file for the IHC application as this was reported to us by a customer. I would recommend a standard IHC protocol for staining and a dilution of 1:100. I would recommend antigen retrieval using a heat mediated citrate buffer at a pH 6.0. You can find the general protocol information that our lab recommends here. I hope your customer finds this information useful and again I apologize we do not have any images to send over, but do guarantee this antibody to work for all species and applications listed. The customer may need to directly conjugate the primary antibody to avoid high background with their anti-mouse secondary detecting the mouse samples.
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