Nrf1 Antibody (2F9) - Azide and BSA Free

Novus Biologicals | Catalog # H00004899-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F9

Format

Azide and BSA Free
View all Nrf1 Primary Antibodies »

Product Specifications

Immunogen

NRF1 (NP_005002, 201 a.a. ~ 285 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQ

Specificity

NRF1 - nuclear respiratory factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Nrf1 Antibody (2F9) - Azide and BSA Free

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01]

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01]

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Nrf1 Antibody (2F9) [H00004899-M01]

Immunohistochemistry-Paraffin: Nrf1 Antibody (2F9) [H00004899-M01]

Immunohistochemistry-Paraffin: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of monoclonal antibody to NRF1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Western Blot: Nrf1 Antibody (2F9) [H00004899-M01]

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01]

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( Cat # H00004899-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody (M01), clone 2F9 (Cat # H00004899-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: Nrf1 Antibody (2F9) [H00004899-M01]

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01]

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in HeLa.
Western Blot: Nrf1 Antibody (2F9) [H00004899-M01]

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01]

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in human Skeletal muscle.
ELISA: Nrf1 Antibody (2F9) [H00004899-M01]

ELISA: Nrf1 Antibody (2F9) [H00004899-M01]

ELISA: Nrf1 Antibody (2F9) [H00004899-M01] - Detection limit for recombinant GST tagged NRF1 is approximately 0.03ng/ml as a capture antibody.

Applications for Nrf1 Antibody (2F9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against tissue lysate and recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nrf1

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Long Name

NF-E2-related Factor 1

Alternate Names

HBZ17, LCR-F1, NFE2L1, TCF11

Entrez Gene IDs

4899 (Human)

Gene Symbol

NRF1

OMIM

600879 (Human)

UniProt

NP_005002 (Human)

Additional Nrf1 Products

Product Documents for Nrf1 Antibody (2F9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Nrf1 Antibody (2F9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nrf1 Antibody (2F9) - Azide and BSA Free

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Protocols

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FAQs

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