Pax6 Antibody (MSVA-706M)

Novus Biologicals | Catalog # NBP3-57271

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSVA-706M
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Product Specifications

Immunogen

Recombinant human PAX6 fragment Swissprot P26367

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Pancreas: A strong PAX6 positivity should be seen in the cells of islets of Langerhans..

Negative Control
Negitve control:Tonsil: PAX6 immunostaining should be absent in all cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Pax6 Antibody (MSVA-706M)

Pax6 Antibody (MSVA-706M)

Immunohistochemistry-Paraffin:Pax6 Antibody (MSVA-706M)NBP3-57271

Pancreatic neuroendocrine tumor with strong predominantly nuclear Pax6 positivity of all tumor cells
Pax6 Antibody (MSVA-706M)

Immunohistochemistry-Paraffin:Pax6 Antibody (MSVA-706M)NBP3-57271

Pancreas with intense nuclear Pax6 staining of islet cells scaled
Pax6 Antibody (MSVA-706M)

Immunohistochemistry-Paraffin:Pax6 Antibody (MSVA-706M)NBP3-57271

Cerebellum with nuclear Pax6 staining of granule cells scaled
Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Cancer Tissue Gallery

Pax6 Cancer Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Cancer Tissue Gallery

Pax6 Cancer Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Cancer Tissue Gallery

Pax6 Cancer Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Cancer Tissue Gallery

Pax6 Cancer Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Cancer Tissue Gallery

Pax6 Cancer Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Normal Tissue Gallery

Pax6 Normal Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Normal Tissue Gallery

Pax6 Normal Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Normal Tissue Gallery

Pax6 Normal Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Normal Tissue Gallery

Pax6 Normal Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Normal Tissue Gallery

Pax6 Normal Tissue Gallery

Pax6 Antibody (MSVA-706M) Pax6 Antibody (MSVA-706M) Pax6 Normal Tissue Gallery

Pax6 Normal Tissue Gallery

Applications for Pax6 Antibody (MSVA-706M)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Pax6

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Gene Symbol

PAX6

Additional Pax6 Products

Product Documents for Pax6 Antibody (MSVA-706M)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Pax6 Antibody (MSVA-706M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Pax6 Antibody (MSVA-706M)

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  • Q: What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?

    A:

    By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

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