Periostin/OSF-2 Antibody (24GB930)

Novus Biologicals | Catalog # NBP3-50575

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB930
View all Periostin/OSF-2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Periostin/OSF-2 (Uniprot: Q15063)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Periostin/OSF-2 Antibody (24GB930)

Periostin/OSF-2 Antibody (24GB930)

Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody (24GB930)[NBP3-50575] -

Immunocytochemical staining of HepG2 cells with Periostin/OSF-2 antibody (1:1,000). Nuclei were stained blue with DAPI; Periostin/OSF-2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Periostin/OSF-2 Antibody (24GB930)

Flow Cytometry: Periostin/OSF-2 Antibody (24GB930)[NBP3-50575] -

Flow cytometric analysis of Periostin/OSF-2expression in HepG2 cells using Periostin/OSF-2 antibody (1:2,000). Green, isotype control; red, Periostin.
Periostin/OSF-2 Antibody (24GB930)

Western Blot: Periostin/OSF-2 Antibody (24GB930)[NBP3-50575] -

Western blotting analysis using Periostin/OSF-2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Periostin/OSF-2 Antibody (24GB930)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Gene Symbol

POSTN

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody (24GB930)

Certificate of Analysis

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Product Specific Notices for Periostin/OSF-2 Antibody (24GB930)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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