Periostin/OSF-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82472

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Canine

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Periostin/OSF-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GLFINHYPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSESIMGGAVFETLEG

Reactivity Notes

Use in Canine reported in scientific literature (PMID: 32354887).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Periostin/OSF-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining in human skin and pancreas tissues using anti-POSTN antibody. Corresponding POSTN RNA-seq data are presented for the same tissues.
Western Blot: Periostin/OSF-2 Antibody [NBP1-82472]

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472]

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human skin shows high expression.
Knockdown Validated: Periostin/OSF-2 Antibody [NBP1-82472]

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472]

Periostin-OSF-2-Antibody-Knockdown-Validated-NBP1-82472-img0009.jpg
Periostin/OSF-2 Antibody

Immunohistochemistry: Periostin/OSF-2 Antibody [NBP1-82472] -

nbp1-82472_rabbit-periostin-osf-2-pab-20920231742123.jpg
Periostin/OSF-2 Antibody

Immunohistochemistry: Periostin/OSF-2 Antibody [NBP1-82472] -

Serial sections of non-small cell lung carcinoma (NSCLC) immunostained for (A) periostin (POSTN), (B) alpha -smooth muscle actin ( alpha -SMA), (C) podoplanin (D2-40), and (D) vimentin in cancer-associated fibroblasts (CAFs). Magnification 50×. Bar = 200 µm.
Periostin/OSF-2 Antibody

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] -

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - The efficacy of shRNA lentiviral particles transfection. (A) Silencing of periostin (POSTN) expression was confirmed by Western blot analysis. beta -actin was used as an internal control. (B) Confocal images & fluorescence analysis showing the expression of POSTN in control A549.CTRL cells & A549.shRNA cells (*** p < 0.001). Objective 60×/1.40 Oil; pinhole airy 1.25. Bar = 20 µm. (C) The expression of POSTN mRNA in control cells (A549.CTRL) & the cells representing the loss-of-function phenotype (A549.shRNA). Relative expression (RQ) of the POSTN gene was normalized against the expression of ACTB (*** p < 0.001). RQ, relative quantification. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35163164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Periostin/OSF-2 Antibody

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] -

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - Characteristics of lung cancer cell lines according to periostin (POSTN) expression. (A) Comparison of POSTN expression levels detected by Western blot & (B) by confocal microscopy (fluorescence intensity) in different lung cancer cell lines (A549, NCI-H522, NCI-H1703), (p > 0.05). Objective 60×/1.40 Oil; pinhole airy 1.25. Bar = 20 µm. (C) Immunohistochemical expression level of periostin (POSTN) in the tissue of particular non-small cell lung carcinoma (NSCLC) subtypes: squamous cell carcinoma (SCC) & adenocarcinoma (AC) (p > 0.05). IRS, immunoreactive score. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35163164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Periostin/OSF-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-P, Retrieval method: HIER pH6.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Entrez Gene IDs

10631 (Human); 50706 (Mouse)

Gene Symbol

POSTN

UniProt

Q15063

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Periostin/OSF-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Periostin/OSF-2 Antibody - BSA Free

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Protocols

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