PI 3-Kinase p110 delta Antibody (PIK3CD/4639)
Novus Biologicals | Catalog # NBP3-23783
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # PIK3CD/4639
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Product Specifications
Immunogen
Recombinant fragment of human PI 3-Kinase p110 delta protein (around aa 520-720) (exact sequence is proprietary)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Non-hazardous. No MSDS required.
Non-hazardous. No MSDS required.
Scientific Data Images for PI 3-Kinase p110 delta Antibody (PIK3CD/4639)
Immunohistochemistry-Paraffin: PI 3-Kinase p110 delta Antibody (PIK3CD/4639) [NBP3-23783] -
Formalin-fixed, paraffin-embedded human lymph node stained with PI 3-Kinase p110 delta antibody (PIK3CD/4639). Inset: PBS instead of primary antibody; secondary only negative control.
Protein Array: PI 3-Kinase p110 delta Antibody (PIK3CD/4639) [NBP3-23783] -
Analysis of Protein Array containing more than 19,000 full-length human proteins using PI 3-Kinase p110 delta antibody (PIK3CD/4639)
Western Blot: PI 3-Kinase p110 delta Antibody (PIK3CD/4639) [NBP3-23783] -
Western blot analysis of THP-1 cell lysate using PI 3-Kinase p110 delta antibody (PIK3CD/4639)Applications for PI 3-Kinase p110 delta Antibody (PIK3CD/4639)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Western Blot
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: PI 3-Kinase p110 delta
Long Name
Protein Tyrosine Phosphatase 1D
Alternate Names
PI 3Kinase p110 delta, PIK3CD
Gene Symbol
PIK3CD
Additional PI 3-Kinase p110 delta Products
Product Documents for PI 3-Kinase p110 delta Antibody (PIK3CD/4639)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PI 3-Kinase p110 delta Antibody (PIK3CD/4639)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Common Cytokine Receptor Gamma-Chain Family Signaling Pathways
IL-2 Signaling Pathways
IL-4 Signaling Pathways
IL-7 Signaling Pathways
IL-9 Signaling Pathways
IL-15 Signaling Pathways
IL-21 Signaling Pathways
mTOR Signaling Pathway
Notch Signaling Pathways
TGF-beta Signaling Pathways
VEGF - VEGF R2 Signaling Pathways
