PKC gamma Antibody (24GB4495)

Name: PKC gamma Antibody (24GB4495)
Brand: Novus Biologicals
SKU: NBP3-50800
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-50800

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4495

View all PKC gamma Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human PKC gamma (Uniprot: P05129)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC gamma Antibody (24GB4495)

Immunocytochemistry/ Immunofluorescence: PKC gamma Antibody (24GB4495)[NBP3-50800] -

Immunocytochemical staining of HepG2 cells with PKC gamma antibody(1:1,000). Nuclei were stained blue with DAPI; PKC gamma was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Flow Cytometry: PKC gamma Antibody (24GB4495)[NBP3-50800] -

Flow cytometric analysis of PKC gammaexpression in HepG2 cells using PKC gamma antibody (1:2,000). Green, isotype control; red, PKC gamma.

Western Blot: PKC gamma Antibody (24GB4495)[NBP3-50800] -

Western blotting analysis using PKC gamma antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for PKC gamma Antibody (24GB4495)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKC gamma

Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1.

Long Name

Protein Kinase C gamma

Alternate Names

PKCC, PKCG, PRKCG, SCA14

Gene Symbol

PRKCG

Additional PKC gamma Products

Product Documents for PKC gamma Antibody (24GB4495)

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Certificate of Analysis

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Product Specific Notices for PKC gamma Antibody (24GB4495)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.