Rac1 Antibody (JM11-29)

Name: Rac1 Antibody (JM11-29)
Brand: Novus Biologicals
SKU: NBP2-67091
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-67091

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM11-29 expressed in HEK293

View all Rac1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length protein of Human Rac1 aa 1-192 / 192. (SwissProt: P63000 Human; SwissProt: P15153 Human; SwissProt: P60763 Human; SwissProt: P63001 Mouse; SwissProt: Q6RUV5 Rat)

Localization

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rac1 Antibody (JM11-29)

Western Blot: Rac1 Antibody (JM11-29) [NBP2-67091]

Western Blot: Rac1 Antibody (JM11-29) [NBP2-67091] - Analysis of Rac1-2-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: MCF-7 cell lysateLane 2: PC-12 cell lysate

Immunocytochemistry/ Immunofluorescence: Rac1 Antibody (JM11-29) [NBP2-67091]

Immunocytochemistry/Immunofluorescence: Rac1 Antibody (JM11-29) [NBP2-67091] - Staining RAC1/2/3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Rac1 Antibody (JM11-29) [NBP2-67091]

Immunohistochemistry-Paraffin: Rac1 Antibody (JM11-29) [NBP2-67091] - Analysis of paraffin-embedded human tonsil tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.

Immunocytochemistry/ Immunofluorescence: Rac1 Antibody (JM11-29) [NBP2-67091]

Immunocytochemistry/Immunofluorescence: Rac1 Antibody (JM11-29) [NBP2-67091] - Staining RAC1/2/3 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: Rac1 Antibody (JM11-29) [NBP2-67091]

Immunocytochemistry/Immunofluorescence: Rac1 Antibody (JM11-29) [NBP2-67091] - Staining RAC1/2/3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Rac1 Antibody (JM11-29) [NBP2-67091]

Immunohistochemistry-Paraffin: Rac1 Antibody (JM11-29) [NBP2-67091] - Analysis of paraffin-embedded human spleen tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.

Western Blot: Rac1 Antibody (JM11-29) [NBP2-67091]

Western blot analysis of MCF-7 cell lysates with NBP2-67091 at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67091, 1/2,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 21.5kDa Observed band size: 21kDa

Western Blot: Rac1 Antibody (JM11-29) [NBP2-67091]

Western blot analysis of mouse brain tissue lysates with NBP2-67091 at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67091, 1/2,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 21.5kDa Observed band size: 21kDa

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Applications for Rac1 Antibody (JM11-29)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rac1

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

Long Name

Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, small GTP binding Protein Rac1)

Alternate Names

p21-Rac1, TC-25

Gene Symbol

RAC1

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Product Specific Notices for Rac1 Antibody (JM11-29)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.