Rac1 Antibody

Novus Biologicals | Catalog # NB100-91266

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Rac1 Primary Antibodies »

Product Specifications

Immunogen

This Rac1 antibody was developed against a synthetic peptide from amino acid region 100-150 as a part of human Rac1 conjugated to blue carrier protein.

Reactivity Notes

Predicted cross-reactivity based on antigen identity: Guinea pig, Bovine, Xenopus, Canine, Zebrafish, Chicken.

Specificity

Detects both RAC1 (Gene ID: 5879, UniProt: P63000) and RAC2 (Gene ID: 5880, UniProt: P15153).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rac1 Antibody

Western Blot: Rac1 Antibody [NB100-91266]

Western Blot: Rac1 Antibody [NB100-91266]

Western Blot: Rac1 Antibody [NB100-91266] - WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution: 1:1000 incubated overnight at 4C.
Immunocytochemistry/ Immunofluorescence: Rac1 Antibody [NB100-91266]

Immunocytochemistry/ Immunofluorescence: Rac1 Antibody [NB100-91266]

Immunocytochemistry/Immunofluorescence: Rac1 Antibody [NB100-91266] - Mouse Brain (cerebral cortex). ICC/IF image submitted by a verified customer review.
Immunohistochemistry: Rac1 Antibody [NB100-91266]

Immunohistochemistry: Rac1 Antibody [NB100-91266]

Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat spinal cord using Rabbit antibody to RAC1, RAC2 (100-150) at 1:1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: Rac1 Antibody [NB100-91266]

Immunohistochemistry: Rac1 Antibody [NB100-91266]

Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat spinal cord (ventral horn) using Rabbit antibody to RAC1, RAC2 whole serum at 1:1000 dilution. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: Rac1 Antibody [NB100-91266]

Immunohistochemistry: Rac1 Antibody [NB100-91266]

Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat ligated sciatic nerve using Rabbit antibody to RAC1, RAC2 whole serum at 1:1000 dilution. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).
Rac1 Antibody

Western Blot: Rac1 Antibody [NB100-91266] -

Melatonin injection restores renal cortical fibrosis in a CKD mouse model via increased expression of miR-4516. (A) Hematoxylin and eosin (H&E) staining was performed on kidney sections from a CKD mouse model following melatonin injection, or melatonin inhibition with miR-4516 inhibitor (scale bar = 1000 μm). (B,C) Expression of miR-4516 and ITGA9 was detected in the kidney cortex in each group by qPCR (n = 3). (D–F) Western blot analysis for ITGA9, Rac1, RhoA, CDC42, collagen type 1, and fibronectin expression using samples from the kidney cortex of each mouse model group (n = 3). Protein levels were determined by densitometry relative to alpha -tubulin. The values represent mean +/- SEM. * p < 0.05, ** p < 0.01 vs. healthy kidney, ##p < 0.01 vs. phosphate buffered saline (PBS), $$p < 0.01 vs. melatonin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32727098), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Rac1 Antibody

Western Blot: Rac1 Antibody [NB100-91266] -

Cytoskeleton reorganization and ITGA9-Rho GTPase signaling pathways are activated due to decreased miR-4516 expression following P-cresol exposure. (A,B) Expression of miR-4516 and ITGA9 was detected in human proximal tubular epithelial (TH1) cells with P-cresol (0.1, 0.25, and 0.5 mM) or indoxyl sulfate (0.2, 0.4, and 0.8 mM) exposure for 72 h (n = 3). The values represent mean +/- SEM. * p < 0.05, ** p < 0.01 vs. control. (C,D) Western blot analysis for ITGA9, Rac1, RhoA, and CDC42 in TH1 cells after exposure to various doses of P-cresol (0, 0.1, 0.25, and 0.5 mM) for 72 h (n = 3). Protein expression was determined by densitometry relative to beta -actin. The values represent mean +/- SEM. ** p < 0.01 vs. control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32727098), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Rac1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:1000 - 1:2000

Immunohistochemistry-Frozen

1:1000 - 1:2000

Immunohistochemistry-Paraffin

1:1000 - 1:2000

Western Blot

1:1000-1:2000
Application Notes
This Rac1 antibody is validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NB100-91266 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rac1

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. It is regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Rac 1: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. Rac 2 is Hematopoietic specific. Defects in Rac 2 are the cause of neutrophil immunodeficiency syndrome.

Long Name

Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, small GTP binding Protein Rac1)

Alternate Names

p21-Rac1, TC-25

Entrez Gene IDs

5879 (Human); 19353 (Mouse); 363875 (Rat)

Gene Symbol

RAC1

UniProt

P63000 (Human)

Additional Rac1 Products

Product Documents for Rac1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Rac1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons
PDGF Family

Customer Reviews for Rac1 Antibody (1)

4 out of 5
1 Customer Rating
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  • Rac1 Antibody
    Name: Chansol Hurr
    Application: Immunohistochemistry
    Sample Tested: Mouse brain and Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 05/11/2017
    Rac1 Antibody NB100-91266

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Protocols

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