Rat Nogo-A aa 2-172 Antibody

Detection of Rat Nogo-A by Western Blot

RT-PCR analysis and western blot analysis of Nogo-A mRNA and protein production in the hippocampus after TBI. (A) The PCR products from Nogo-A transcription after TBI with the yield of actin as an internal control. The sampling times after TBI are shown on the top (n = 4 in each group). (B) Quantification of Nogo-A mRNA expression by semiquantitative densitometry in conjunction with AlphaEase software (Alpha Innotech Corp.). (C) Time course of Nogo-A protein production after TBI, internal control: beta -actin. Times shown on top represent hours after injury. (D) Quantification of Nogo-A protein by semiquantitative densitometry in conjunction with AlphaEase software (Alpha Innotech Corp.). The data are presented as ratios related to the sham group. Bars represent the means ± SEM values. *p < 0.05 is considered significantly different from sham values using the Mann–Whitney U test. SEM, standard error of the mean; TBI, traumatic brain injury. Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Rat Nogo-A by Western Blot

Effects of Nogo-A irrelevant control and antisense oligodeoxynucleotides on hippocampal Nogo-A expression after TBI. (A) RT-PCR analysis of Nogo-A mRNA transcription level. Actin transcription was used as an internal control. (B) The expression of Nogo-A was quantified by densitometry and compared with the data from rats injected with saline (sham), which was normalized to 100%. (C) Western blot analysis of Nogo-A protein level; beta -actin was used as an internal control. (D) Quantification of Nogo-A protein by semiquantitative densitometry in conjunction with AlphaEase software (Alpha Innotech Corp.). The data are presented compared with the sham group. The data are represented as the means ± SEM values (n = 6). *p < 0.05 was considered significantly different from the sham value using the Mann-Whiney U test, and #p < 0.05 was considered significantly different from the TBI with sense values using the Mann-Whiney U test. SEM, standard error of the mean; TBI, traumatic brain injury. Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Rat Nogo-A by Western Blot

Effects of indomethacin administration on Nogo-A expression. Animals were in one of four groups: sham (no TBI), TBI treatment (TBI eight hours), TBI combined with vehicle administration (TBI + vehicle), and TBI combined with indomethacin administration (TBI + indomethacin). (A) RT-PCR analysis of the expression of Nogo-A among different groups along with the analysis of beta -actin transcription as an internal control. (B) Quantification of Nogo-A expression. (C) Western blot analysis of the expression of Nogo-A among different groups along with the analysis of beta -actin as an internal control. (D) Quantification of Nogo-A expression. Bars represent means ± SEM values (n = 5). *P <0.05 is considered significantly different from the sham value using the Mann–Whitney U test and #P <0.05 is considered significantly different from the TBI value using the Mann–Whitney U test. SEM, standard error of the mean; TBI, traumatic brain injury. Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094…), licensed under a CC-BY license. Not internally tested by R&D Systems.