Recombinant Human CD117/c-kit Fc Biotinylated Protein, CF New

Catalog #: BT10961 Datasheet / COA / SDS

Fc Chimera

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BT10961-050

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Biotinylated Recombinant Human CD117/c-kit Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD117/c-kit Fc Biotinylated Protein, CF Summary

R&D Systems CHO-derived Recombinant Human CD117/c-kit Fc Biotinylated Protein (BT10961)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
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Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD117/c-kit Fc Chimera (Catalog # BT10961) binds Recombinant Human SCF/c-kit Ligand (Catalog # BT-SCF) with an ED₅₀ of 0.400-4.00 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD117/c-kit protein

Human CD117/c-kit (Gln26-Thr520) Accession # P10721.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

N-terminal Sequence
Analysis

Gln26

Structure / Form

Disulfide-linked homodimer

Biotinylated via amines

Predicted Molecular Mass

82 kDa

SDS-PAGE

110-120 kDa, under reducing conditions.

Product Datasheets

BT10961

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT10961

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Biotinylated Recombinant Human CD117/c-kit Fc Chimera Protein (Catalog # BT10961) binds Recombinant Human SCF/c-kit Ligand (BT-SCF) with an ED₅₀ of 0.400-4.00 μg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CD117/c-kit Fc Chimera Protein (Catalog # BT10961) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-120 kDa and 220-240 kDa, respectively.

Reconstitution Calculator

Background: CD117/c-kit

Stem cell factor receptor (SCFR), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that acts as the receptor for the cytokine SCF. Human SCFR is expressed in embryonic stem cells and is involved in regulation of cell survival and proliferation, hematopoiesis, gametogenesis, and melanogenesis (1-3). Mature human SCFR consists of an extracellular domain (ECD) with five tandem immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain. Within the ECD, human SCFR shares 73% and 76% amino acid sequence identity with mouse and rat SCFR, respectively. Several SCFR isoforms, produced by alternative mRNA splicing, have been identified in humans and can be characterized by the presence or absence of a tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (4, 5). Binding of SCFR to SCF triggers receptor dimerization and activates downstream signaling (6). SCF is a primary growth and activation factor for mast cells and eosinophils and SCFR expression on mast cells enables them to infiltrate SCF‑secreting tumors where they promote tumor growth and induce local immune suppression (7, 8). SCFR is up regulated on dendritic cells by Th2- or Th17-biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (9). SCFR protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (10, 11). Mutations or deletions of SCFR cause a wide variety of malignancies as well as pigmentation disorders and sterility (12).

References

Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
Lennartsson, J. and Rönnstrand L. (2012) Physiol Rev. 92:1619.
Caruana, G. et al. (1999) Oncogene 18:5573.
Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
Huang, B. et al. (2008) Blood 112:1269.
Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.

Long Name

Stem Cell Factor Receptor

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Alternate Names

CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein