Recombinant Human CD38 Fc Chimera Biotinylated Protein, CF New

Catalog #: BT10920 Datasheet / COA / SDS

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BT10920-050

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Biotinylated Recombinant Human CD38 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD38 Fc Chimera Biotinylated Protein, CF Summary

R&D Systems HEK293-derived Recombinant Human CD38 Fc Chimera Biotinylated Protein (BT10920)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
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Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD38 Fc Chimera binds to Human CD38 Antibody (Catalog # MAB2404) with an ED₅₀ of 1.00-10.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CD38 protein

Human CD38 (Val43-Ile300) Accession # P28907.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

N-terminal Sequence
Analysis

Val43

Structure / Form

Disulfide-linked homodimer, Biotinylated via amines

Predicted Molecular Mass

32 kDa

SDS-PAGE

64-76 kDa, under reducing conditions.

Product Datasheets

BT10920

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT10920

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD38 Fc Chimera Protein (Catalog # BT10920) binds to Human CD38 Antibody (MAB2404) with an ED₅₀ of 1.00-10.0 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CD38 Fc Chimera Protein (Catalog # BT10920) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-76 kDa and 130-150 kDa, respectively.

Reconstitution Calculator

Background: CD38

CD38, also known as ADP-ribosyl cyclase, converts NAD(P)+ into three separate products with calcium mobilizing ability: cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is a Type II transmembrane glycoprotein composed of an intracellular domain, a single transmembrane helix domain, and a large extracellular domain that contains the catalytic site (2). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (3,4). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells (5,6). CD38 is also reported to bind as a receptor to trigger signaling cascades (7,8). Through both mechanisms, CD38 influences proliferation and trafficking (8,9). CD38 is used as a marker for poor prognosis in chronic lymphocytic leukemia and multiple myeloma and is an attractive cancer immunotherapy drug target (8-11).

References

Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
Liu, Q. et al. (2005) Structure. 13:1331.
Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
Sun, L. et al. (1999) J. Cell Biol. 146:1161.
Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
Deaglio, S. (2000) Chem. Immunol. 75:99.
Chillemi,A. et al. (2013) Mol. Med. 19:99.
Vaisitti, T. et al. (2015) Leukemia. 29:356.
Atanackovic, D. et al. (2016) Oncoimmunology. 5:e1217374.
Tai, Y-T. et al. (2017) Oncotarget. 8:112166.

Long Name

Cluster of Differentiation 38

Entrez Gene IDs

952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)

Alternate Names

2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20); 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl Cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase; cADPr hydrolase 1; CD_antigen: CD38; CD38 antigen (p45); CD38 antigen; CD38 molecule; CD38; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; Cyclic ADP-ribose Hydrolase; EC 2.4.99.20; EC 3.2.2.6; EC:3.2.2.6; NAD(+) nucleosidase; T10