Recombinant Human TGF-beta RII Fc Avi-tag Biotin Protein, CF

Name: Recombinant Human TGF-beta RII Fc Avi-tag Biotin Protein, CF
Brand: R&D Systems
SKU: AVI341
Price: 794 USD
Availability: InStock

R&D Systems | Catalog # AVI341

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems HEK293-derived Recombinant Human TGF-beta RII Fc Avi-tag Biotin Protein (AVI341)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P37173.2

Structure / Form

Biotinylated via Avi-tag.

Conjugate

Biotin

Applications

Bioactivity

View all TGF-beta RII Proteins and Enzymes »

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TGF-beta RII protein

Human TGF-beta RII (Thr23-Asp159) Accession # P37173.2	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr23

Predicted Molecular Mass

44 kDa

SDS-PAGE

56-72 kDa, under reducing conditions.

Activity

When Recombinant Human TGF-beta 1 (Catalog # 7754-BH) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human TGF-beta RII Fc Chimera Avi-tag (Catalog # AVI341) binds with an ED₅₀ of 0.2-1.6 ng/mL.

Scientific Data Images for Recombinant Human TGF-beta RII Fc Avi-tag Biotin Protein, CF

Biotinylated Recombinant Human TGF‑ beta RII Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human TGF‑ beta 1 (7754-BH) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human TGF-beta RII Fc Chimera Avi-tag (Catalog # AVI341) binds with an ED₅₀ of 0.2-1.6 ng/mL.

Biotinylated Recombinant Human TGF‑ beta RII Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human TGF‑ beta RII Fc Chimera Avi-tag Protein (Catalog # AVI341) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 56-72 kDa and 110-140 kDa, respectively.

Formulation, Preparation, and Storage

AVI341

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors. The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta. Our Avi-tag Biotinylated TGF-beta RII Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.