RNase L Antibody (2E9) - BSA Free
Novus Biologicals | Catalog # NB100-351
![Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]](https://resources.rndsystems.com/images/products/RNase-L-Antibody-2E9-BSA-Free-Western-Blot-NB100-351-img0010.jpg "Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Hamster, Mouse (Negative), Primate, Rat (Negative)
Cited:
Human
Applications
Validated:
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E9
Format
BSA Free
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Product Specifications
Immunogen
Purified full-length recombinant human RNaseL produced in insect cells. [UniProt# Q05823]
Reactivity Notes
This antibody does not react with mouse or rat.
Localization
Cytoplasmic and Mitochondrial.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RNase L Antibody (2E9) - BSA Free
Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]
RNase-L-Antibody-2E9-BSA-Free-Western-Blot-NB100-351-img0010.jpg![Immunohistochemistry: RNase L Antibody (2E9) - BSA Free [NB100-351]](https://resources.rndsystems.com/images/products/RNase-L-Antibody-2E9-Immunohistochemistry-NB100-351-img0006.jpg "Immunohistochemistry: RNase L Antibody (2E9) - BSA Free [NB100-351]")
Immunohistochemistry: RNase L Antibody (2E9) - BSA Free [NB100-351]
Immunohistochemistry: RNase L Antibody (2E9) [NB100-351] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain.![Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]](https://resources.rndsystems.com/images/products/RNase-L-Antibody-2E9-Western-Blot-NB100-351-img0008.jpg "Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]")
Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]
Western Blot: RNase L Antibody (2E9) [NB100-351] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug)![Knockout Validated: RNase L Antibody (2E9) - BSA Free [NB100-351]](https://resources.rndsystems.com/images/products/RNase-L-Antibody-2E9-BSA-Free-Knockout-Validated-NB100-351-img0009.jpg "Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351]")
Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] -
Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - dsRNA-induced SGs require G3BP1 & G3BP2, whereas RLBs do not.A, immunoblot for G3BP1, RNase L, GAPDH in parental (WT), RL-KO, G3BP-KO, & RL/G3BP-KO U-2 OS cells. B, immunoblot for G3BP1 & G3BP2 in RL-KO & RL/G3BP-KO U-2 OS cells. C, IF for G3BP1 & PABPC1 4 h post-poly(I·C) transfection or treatment with 500 μm sodium arsenite (SA) in the indicated cell lines. D, similar to C but enlarged to show RLB formation in U-2 OS-G3BP1/2-KO cells. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31896577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for RNase L Antibody (2E9) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:1000-1:4000 (ECL)
Application Notes
This RNase L (2E9) antibody is useful for ELISA, Western Blot and Immunohistochemistry on paraffin-embedded sections. By WB, this antibody recognizes a band at ~83 kDa, representing RNase L. A low-molecular mass, 37 kDa, isoform of RNase L has been described in peripheral blood mononuclear cell extracts. In IHC-P, staining was observed in the cytoplasm of human colon cells. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNase L
Alternate Names
2-5A-dependent ribonuclease, 2-5A-dependent RNase, 2'-5'-oligoisoadenylate synthetase-dependent, EC 3.1.26.-, HPC1, interferon-induced 2-5A-dependent RNase, MGC104972, MGC133329, PRCA1, prostate cancer 1, Ribonuclease 4, Ribonuclease L, ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent), RNase L, RNS4DKFZp781D08126
Gene Symbol
RNASEL
Additional RNase L Products
Product Documents for RNase L Antibody (2E9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RNase L Antibody (2E9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for RNase L Antibody (2E9) - BSA Free
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Protocols
View specific protocols for RNase L Antibody (2E9) - BSA Free (NB100-351):
RNase L Antibody (2E9):
Western Blot
1. Proteins are separated on a 10% SDS-PAGE gel.
2. Proteins are transferred to Immobilon-P membranes (Millipore Co.).
3. Following the protein transfer, the membrane is blocked with PBS-T [PBS + 0.07% Tween-20]+ 5% NFDM.
4. Anti-RNase L [cat# NB 100-351] is diluted 1:1,000, in blocking buffer and incubated for 2 hour at room temperature, gently shaking.
5. The membrane is then washed, 3 times with PBS-T, 5 minutes each.
6. Secondary antibody is incubated for 1 hour at room temperature, gently shaking.
7. The membrane is then washed, 4 times with PBS-T, 5 minutes each.
8. Membrane is developed using ECL reagents.
NOTE: Cell extracts of insect cells expressing human RNase L (0.1 ug) and Hey 1B (100 ug) were used as positive controls for this antibody.
Western Blot
1. Proteins are separated on a 10% SDS-PAGE gel.
2. Proteins are transferred to Immobilon-P membranes (Millipore Co.).
3. Following the protein transfer, the membrane is blocked with PBS-T [PBS + 0.07% Tween-20]+ 5% NFDM.
4. Anti-RNase L [cat# NB 100-351] is diluted 1:1,000, in blocking buffer and incubated for 2 hour at room temperature, gently shaking.
5. The membrane is then washed, 3 times with PBS-T, 5 minutes each.
6. Secondary antibody is incubated for 1 hour at room temperature, gently shaking.
7. The membrane is then washed, 4 times with PBS-T, 5 minutes each.
8. Membrane is developed using ECL reagents.
NOTE: Cell extracts of insect cells expressing human RNase L (0.1 ug) and Hey 1B (100 ug) were used as positive controls for this antibody.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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