Smad4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24951

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Smad4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against synthetic peptides corresponding to amino acids 186-223 and amino acids 509-544 of human SMAD4 (GenBank Accession No. NP_005350.1). These peptide sequences are specific to SMAD4, and have high homology to SMAD4 in multiple species.

Reactivity Notes

Bovine reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smad4 Antibody - BSA Free

Western Blot: Smad4 Antibody [NBP2-24951]

Western Blot: Smad4 Antibody [NBP2-24951]

Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Smad4 Antibody [NBP2-24951]

Immunocytochemistry/ Immunofluorescence: Smad4 Antibody [NBP2-24951]

Immunocytochemistry/Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMAD4 Antibody NBP2-24951 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951]

Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951]

Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Apical cytoplasmic staining was observed in both glandular and epithelial cells.

Applications for Smad4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 5 ug/mL

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1 - 3 ug/mL

Reviewed Applications

Read 3 reviews rated 3.7 using NBP2-24951 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad4

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Gene Symbol

SMAD4

UniProt

Q13485

Additional Smad4 Products

Product Documents for Smad4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Smad4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Smad4 Antibody - BSA Free

Customer Reviews for Smad4 Antibody - BSA Free (3)

3.7 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Smad4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: eye
    Species: Human
    Verified Customer | Posted 05/26/2021
    gives multiple bands
    used at 1:100 overnight
    Smad4 Antibody - BSA Free NBP2-24951
  • Smad4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse eye
    Species: Mouse
    Verified Customer | Posted 05/26/2021
    works well
    used at 1:100 overnight
    Smad4 Antibody - BSA Free NBP2-24951
  • Smad4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: eye
    Species: Bovine
    Verified Customer | Posted 05/26/2021
    works well
    used at 1:100 overnight
    Smad4 Antibody - BSA Free NBP2-24951

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