SMUG1 Antibody

Novus Biologicals | Catalog # NB100-1414

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SMUG1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence PQAFLLGSIHEPA-C corresponding to N-Terminus according to NP_055126.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMUG1 Antibody

Immunocytochemistry/ Immunofluorescence: SMUG1 Antibody [NB100-1414]

Immunocytochemistry/ Immunofluorescence: SMUG1 Antibody [NB100-1414]

Immunocytochemistry/Immunofluorescence: SMUG1 Antibody [NB100-1414] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: SMUG1 Antibody [NB100-1414]

Flow Cytometry: SMUG1 Antibody [NB100-1414]

Flow Cytometry: SMUG1 Antibody [NB100-1414] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: SMUG1 Antibody [NB100-1414]

Immunocytochemistry/ Immunofluorescence: SMUG1 Antibody [NB100-1414]

Immunocytochemistry/Immunofluorescence: SMUG1 Antibody [NB100-1414] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for SMUG1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000
Application Notes
Use in Western blot has been reported in scientific literature (PMID: 19384898).

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMUG1

SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair.

Long Name

Single-strand Selective Monofunctional Uracil DNA Glycosylase 1

Alternate Names

EC 3.2.2, EC 3.2.2.-, FDG, HMUDG, MGC104370, single-strand selective monofunctional uracil DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase 1, UNG3

Entrez Gene IDs

23583 (Human)

Gene Symbol

SMUG1

UniProt

Q53HV7 (Human)

Additional SMUG1 Products

Product Documents for SMUG1 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for SMUG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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Protocols

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FAQs

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