Sortilin Antibody (CL6526)

Novus Biologicals | Catalog # NBP3-52483

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # CL6526
View all Sortilin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q99523, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Sortilin Antibody (CL6526)

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Staining of human lymph node shows only weak positivity in a subset of lymphoid cells, as expected.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Staining of mouse hippocampus shows strong cytoplasmic positivity in both pyramidal neurons and granular cells.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP3-52483]

Analysis in human cerebral cortex and lymph node tissues. Corresponding RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Sortilin Antibody (CL6526) [NBP3-52483]

Immunocytochemistry/ Immunofluorescence: Sortilin Antibody (CL6526) [NBP3-52483]

Staining of RH-30 cells showing specific staining of the Golgi apparatus in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
Western Blot: Sortilin Antibody (CL6526) [NBP3-52483]

Western Blot: Sortilin Antibody (CL6526) [NBP3-52483]

Analysis in human cell line SK-MEL-30.

Applications for Sortilin Antibody (CL6526)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sortilin

Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.

Alternate Names

Gp95, Ntr3, SORT1

Gene Symbol

SORT1

Additional Sortilin Products

Product Documents for Sortilin Antibody (CL6526)

Certificate of Analysis

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Product Specific Notices for Sortilin Antibody (CL6526)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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