SOX2 Antibody (4G8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80957

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chicken (93%), Monkey (99%), Mouse (96%), Primate (99%), Sheep (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4G8

Format

Azide and BSA Free
View all SOX2 Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human SOX2 (NCBI NP_003097).

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (96%), Chicken (93%), Sheep (99%) and Monkey/Primate species (99%), Zebrafish (87%), Xenopus tropicalis (87%), Xenopus laevis (88%).

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SOX2 Antibody (4G8) - Azide and BSA Free

Western Blot: SOX2 Antibody (4G8)Azide and BSA Free [NBP2-80957]

Western Blot: SOX2 Antibody (4G8)Azide and BSA Free [NBP2-80957]

Western Blot: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein. Image from the standard format of this antibody.
Immunocytochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunocytochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunocytochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human uterine cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate staining in the cytoplasm and nuclei, whereas the stro
Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC-P analysis of SOX2 protein in a section of human kidney using 5 ug/ml concentration of SOX2 antibody (clone 4G8). The representative image shows distinct nuclear and cytoplasmic staining pattern in cells of different types of renal tubules and blood v
Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC analysis of formalin-fixed paraffin-embedded section of normal human colon tissue using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. A weak and diffused cytoplasmic staining was observed in majority of the cells in mucosal laye
Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity for SOX2 protein [Magn
Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity, whereas the stroma was
Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lung cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate immunopositivity of SOX2 protein in the cytoplasm and the
Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human brain using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The representative photomicrograph depicts a cytoplasmic and nuclear immunopositivity of SOX2
Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The liver section depicted a diffused cytoplasmic staining in the hepatocytes [Magnification 4
Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957]

Immunohistochemistry: SOX2 Antibody (4G8) - Azide and BSA Free [NBP2-80957] - IHC-P analysis of SOX2 protein in a section of human renal cell carcinoma using 5 ug/ml concentration of SOX2 antibody (clone 4G8). Granular nuclear and cytoplasmic staining pattern was observed in the carcinoma cells, whereas, a weak to negligible staini

Applications for SOX2 Antibody (4G8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX2

SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. The role of SOX2 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing SOX2 and the transcription factors POU5F1, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Other experiments have shown that iPS cells could be generated using expression plasmids expressing POU5F1, SOX2, KlfF4 and c-Myc, eliminating the need for virus introduction.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Gene Symbol

SOX2

Additional SOX2 Products

Product Documents for SOX2 Antibody (4G8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SOX2 Antibody (4G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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