Src Antibody (5A18) - BSA Free
Novus Biologicals | Catalog # NBP1-19188
![Western Blot: Src Antibody (5A18) [NBP1-19188]](https://resources.rndsystems.com/images/products/Src-Antibody-5A18-Western-Blot-NBP1-19188-img0004.jpg "Western Blot: Src Antibody (5A18) [NBP1-19188]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse Clone # 5A18
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human full length c-Src
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
Src (5A18)
Clonality
Monoclonal
Host
Mouse
Scientific Data Images for Src Antibody (5A18) - BSA Free
![Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]](https://resources.rndsystems.com/images/products/Src-Antibody-5A18-Immunohistochemistry-NBP1-19188-img0002.jpg "Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]")
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human prostate tissue stained with Src, mAb (5A18) at 20 ug/ml.![Western Blot: Src Antibody (5A18) [NBP1-19188]](https://resources.rndsystems.com/images/products/Src-Antibody-5A18-Western-Blot-NBP1-19188-img0003.jpg "Western Blot: Src Antibody (5A18) [NBP1-19188]")
Western Blot: Src Antibody (5A18) [NBP1-19188]
Western Blot: Src Antibody (5A18) [NBP1-19188] - Western blot analysis of Src using 20 ug of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1 ug/ml.![Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]](https://resources.rndsystems.com/images/products/Src-Antibody-5A18-Immunohistochemistry-NBP1-19188-img0001.jpg "Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]")
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human adrenal tissue stained with Src, mAb (5A18) at 20 ug/ml.Applications for Src Antibody (5A18) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1 ug/ml
Application Notes
WB validation from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: tested in A431 -/+ EGF; antibody dilution of 1:50; separated by size
See Simple Western Antibody Database for Simple Western validation: tested in A431 -/+ EGF; antibody dilution of 1:50; separated by size
Reviewed Applications
Read 2 reviews rated 5 using NBP1-19188 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.2) and 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Src
Long Name
v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog
Alternate Names
ASV, c-Src, RSVgp4
Gene Symbol
SRC
UniProt
Additional Src Products
Product Documents for Src Antibody (5A18) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Src Antibody (5A18) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Src Antibody (5A18) - BSA Free
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Customer Reviews for Src Antibody (5A18) - BSA Free (2)
5 out of 5
2 Customer Ratings
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Application: Western BlotSample Tested: Breast cancer cellsSpecies: HumanVerified Customer | Posted 10/11/2018Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Src at Y419 and total Src were detected by western blot.
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Application: Western BlotSample Tested: Hela whole cell lysateSpecies: HumanVerified Customer | Posted 09/12/2012
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling: Oxidative Stress Pathway
MAPK Signaling Pathway: Mitogen Stimulation Pathway
Pathogen or Damage-activated C-Type Lectin Receptor Signaling Pathways
VEGF - VEGF R2 Signaling Pathways
Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling
