Src Antibody Pack

Name: Src Antibody Pack
Brand: Novus Biologicals
SKU: NBP3-20214
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-20214

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species

Human

Applications

Immunohistochemistry, Simple Western, Western Blot

Product Summary for Src Antibody Pack

This pack contains 1 vial each of: AF2685-SP (25 ug), AF2685-SP (25 ug), MAB2685-SP (25 ug), AF3389-SP (1 mL), HAF008 (1 mL).

Product Specifications

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for Src Antibody Pack

Detection of Human Phospho-Src (Y419) in treated MDA-MB-468 and treated HepG2 by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-Src (Y416) in Treated HepG2 lysates by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-Src (Y416) Monoclonal Antibody (Catalog # MAB2685) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Src at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Src in HepG2, MDA-MB-468 and Rat-2 lysates by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Src at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Kit Contents for Src Antibody Pack

AF2685-SP
AF3389-SP
HAF017: Goat IgG HRP-conjugated Antibody
MAB2685-SP
HAF008: Rabbit IgG Horseradish Peroxidase-conjugated Antibody

Formulation, Preparation, and Storage

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Storage

Storage is content dependent.

Background: Src

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Long Name

v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog

Alternate Names

ASV, c-Src, RSVgp4

Gene Symbol

SRC

Additional Src Products

Product Documents for Src Antibody Pack

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Product Specific Notices for Src Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.