TACE/ADAM17 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15281

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Canine (92%), Porcine (93%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TACE/ADAM17 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TACE/ADAM17. The exact sequence is proprietary.

Reactivity Notes

Chicken (83%).

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit TACE/ADAM17 Antibody - BSA Free (NBP2-15281) is a polyclonal antibody validated for use in IHC and WB. Anti-TACE/ADAM17 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TACE/ADAM17 Antibody - BSA Free

Western Blot: TACE/ADAM17 Antibody [NBP2-15281]

Western Blot: TACE/ADAM17 Antibody [NBP2-15281]

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.
Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP2-15281]

Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP2-15281]

Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP2-15281] - Human breast carcinoma dilution: 1:250. ADAM17 antibody [C2C3], C-term dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: TACE/ADAM17 Antibody [NBP2-15281]

Western Blot: TACE/ADAM17 Antibody [NBP2-15281]

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - 30 ug K562 whole cell lysate/extract, 7.5% SDS-PAGE gel, dilution 1:500.
Western Blot: TACE/ADAM17 Antibody [NBP2-15281]

Western Blot: TACE/ADAM17 Antibody [NBP2-15281]

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.
TACE/ADAM17 Antibody

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] -

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TACE/ADAM17 antibody [C2C3], C-term (NBP2-15281) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
TACE/ADAM17 Antibody

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] -

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Wild-type (WT) and ADAM17 knockout (KO) HeLa (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for TACE/ADAM17 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TACE/ADAM17

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq]

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Gene Symbol

ADAM17

UniProt

P78536

Additional TACE/ADAM17 Products

Product Documents for TACE/ADAM17 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TACE/ADAM17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for TACE/ADAM17 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TACE/ADAM17 Antibody - BSA Free

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    • Q: Our customer would like to ensure NBP2-15281 could recognize both active and inactive forms of human ADAM17. Would you please help confirm?
      A: The immunogen which was used to generate our ADAM17 antibody with catalogue number NBP2-15281 was a recombinant fragment corresponding to a region within amino acids 668 and 824 of ADAM17 (Uniprot ID# P78536). These amino acids are found within the chain of the protein, rather than in the signal peptide (amino acids 1-17) or the pro-peptide (amino acids 18-214). Metalloproteinases such as ADAM17 are synthesized as pro-proteins that become active by proteolytic removal of the pro-domain. Since the immunogen sequence lies within the chain you would expect NBP2-15281 to recognise both the active and inactive forms of the protein, although we do not have any specific testing data relating to this.
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