TCF-2/HNF-1 beta Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89680

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TCF-2/HNF-1 beta Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCF-2/HNF-1 beta Antibody - BSA Free

TCF-2/HNF-1 beta Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Immunocytochemistry/ Immunofluorescence: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Staining of human cell line CACO-2 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Nuclear expression of HNF1B (green) in a subset of PDX1 expressing cells (blue) in human pancreas. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Staining of human kidney shows moderate to strong nuclear positivity in renal tubules.
Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Staining of human rectum shows strong nuclear positivity in glandular cells.
TCF-2/HNF-1 beta Antibody - BSA Free Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Analysis in human kidney and lymph node tissues using NBP1-89680 antibody. Corresponding HNF1B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Staining of human lymph node shows no positivity in lymphoid cells as expected.
TCF-2/HNF-1 beta Antibody - BSA Free Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Staining of human rectum shows strong nuclear positivity in glandular cells.
TCF-2/HNF-1 beta Antibody - BSA Free Western Blot: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Western Blot: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Analysis in human kidney tissue.
Simple Western: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Simple Western: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Simple Western: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Electropherogram image(s) of corresponding Simple Western lane view. TCF-2/HNF-1 beta antibody was used at 1:30 dilution on A549 lysate(s).
Simple Western: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Simple Western: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Simple Western: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Simple Western lane view shows a specific band for HNF1B in 0.2 mg/ml of A549 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for TCF-2/HNF-1 beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

1:30

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in A549, separated by Size, antibody dilution of 1:30, apparent MW was 66 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 5 using NBP1-89680 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF-2/HNF-1 beta

TCF2 encodes transcription factor 2, a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1; depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.

Long Name

Transcription Factor 2

Alternate Names

HNF-1 beta, HNF1B, HNF2, LF-B3, MODY5, TCF2, VHNF1

Entrez Gene IDs

6928 (Human)

Gene Symbol

HNF1B

UniProt

P35680

Additional TCF-2/HNF-1 beta Products

Product Documents for TCF-2/HNF-1 beta Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TCF-2/HNF-1 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TCF-2/HNF-1 beta Antibody - BSA Free

Customer Reviews for TCF-2/HNF-1 beta Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human fetal pancreas
    Species: Human
    Verified Customer | Posted 09/25/2014
    Nuclear expression of HNF1B (green) in a subset of PDX1 expressing cells (grey) in developing human pancreas.
    TCF-2/HNF-1 beta Antibody - BSA Free NBP1-89680

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