TDP-43/TARDBP Antibody - BSA Free

Novus Biologicals | Catalog # NB110-55376

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken, Primate, Xenopus, Zebrafish

Cited:

Human, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TDP-43/TARDBP Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide to a C-terminal region [within residues 350-414] of the human TARDBP protein. [Swiss-Prot: Q13148]

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TDP-43/TARDBP Antibody - BSA Free

Western Blot: TDP-43/TARDBP AntibodyBSA Free [NB110-55376]

Western Blot: TDP-43/TARDBP AntibodyBSA Free [NB110-55376]

Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Total protein from HeLa, MCF7 and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TARDBP in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with TDP-43/TARDBP Antibody conjugated to Alexa Fluor 488 (NB110-55376AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-TARDBP at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody [NB110-55376] - Analysis of FFPE human placenta using TDP-43 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.
Western Blot: TDP-43/TARDBP AntibodyBSA Free [NB110-55376]

Western Blot: TDP-43/TARDBP AntibodyBSA Free [NB110-55376]

Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in HeLa WCE.
Western Blot: TDP-43/TARDBP AntibodyBSA Free [NB110-55376]

Western Blot: TDP-43/TARDBP AntibodyBSA Free [NB110-55376]

Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TARDBP cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARDBP.
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in MCF-7 cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and DyLight 550 (Red).
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - Analysis using the DyLight 550 conjugate of NB110-55376. Staining of Human iPS derived neurons, fixed in 4% formaldehyde solution. NB110-55376R was diluted 1:200. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Blue dots in TDP staining is Hoechst.
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376]

TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0013.jpg
TDP-43/TARDBP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody - BSA Free [NB110-55376] -

CWC22 is colocalized with nuclear speckles & upregulated in diabetic DRG sensory neurons. (A) Subcellular distribution of TDP-43 & CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. CWC22 consistently colocalized with a marker protein SC35 of nuclear speckles in sensory neurons. No obvious differences in the subcellular localization of CWC22 were identified in diabetic neurons (not shown) compared with controls. Scale bar: 10 μm. (B) qRT-PCR analysis of Cwc22 mRNA expression in diabetic & control mice. Cwc22 expression was upregulated ∼2.5-fold in diabetic DRGs. *P<0.05, unpaired two-tailed Student's t-test. Data represented as mean±s.e.m. See Cheng et al. (2015) for microarray data indicating rises in Cwc22 expression as reported separately. Image collected & cropped by CiteAb from the following publication (https://journals.biologists.com/dmm/article/10/3/215/2257/Diabetic-poly…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TDP-43/TARDBP Antibody - BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 25853864)

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Western Blot

0.5-2 ug/ml
Application Notes
In Western blot a band is seen at ~45 kDa, representing the human TARDBP protein. In ICC/IF a nuclear signal is present in MCF-7 cells.

Reviewed Applications

Read 1 review rated 3 using NB110-55376 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TDP-43/TARDBP

TARDBP (tar-DNA binding protein of 43) is a nucleic acids binding protein that regulates the process of transcription and splicing. Localized mainly in the nucleus, TARDBP is ubiquitously expressed with higher expression levels in pancreas, placenta, lung, genital tract and spleen, and has been suggested to implicate in microRNA biogenesis, cellular apoptosis as well as proliferation. TARDBP controls CFTR splicing and promotes CFTR exon 9 skipping by associating with UG repeated motifs in polymorphic region near 3'-splice site of this exon leading to aberrant splicing linked to pathological features typical of cystic fibrosis. TARDBP can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Moreover, it stabilizes the low molecular weight neurofilament mRNA through a direct interaction with the 3' UTR. TARDBP was originally identified as a major protein component of the ubiquitin immuno-positive inclusions which are the pathologic substrate of common forms of ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia), and later, it was found that TARDBP gene mutations are inherited in an autosomal dominant manner in familial/sporadic ALS. TARDBP has also been involved in other neurodegenerative such as Alzheimer's and Parkinson's disease.

Long Name

TAR DNA-binding Protein 43

Alternate Names

ALS10, TARDBP, TDP43

Gene Symbol

TARDBP

Additional TDP-43/TARDBP Products

Product Documents for TDP-43/TARDBP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TDP-43/TARDBP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

Citations for TDP-43/TARDBP Antibody - BSA Free

Customer Reviews for TDP-43/TARDBP Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse brain lysates
    Species: Mouse
    Verified Customer | Posted 01/09/2015
    Review_Anti-TDP-43_Novus
    TDP-43/TARDBP Antibody - BSA Free NB110-55376

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Protocols

View specific protocols for TDP-43/TARDBP Antibody - BSA Free (NB110-55376):


Western Blot Protocol

1. Perform SDS-PAGE (4-12% Bis-Tris) on samples to be analyzed, loading 20 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% non-fat dry milk + 1% BSA in TBS, overnight at 4 degrees Celcius.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-TARDBP primary antibody (NB110-55376) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).

Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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