ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, and the response to oxygen and nutrient deprivation. These functions are regulated by the dimerization of ATF4 with the leucine zipper protein FIAT. ATF4 contains a basic DNA-binding domain (aa 280-300) and a leucine zipper domain (aa 306-334).
TMEM119 Antibody (CL8714) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43823
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL8714
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q4V9L6, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse TMEM119 Antibody (CL8714) - Azide and BSA Free (NBP2-76985) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TMEM119 Antibody (CL8714) - Azide and BSA Free
![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://beta-resources.rndsystems.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251211186.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://beta-resources.rndsystems.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251273753.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]
Staining of human cerebellum shows strong membranous positivity in microglia.![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://beta-resources.rndsystems.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-282202512405521.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]
Staining of human cerebral cortex shows strong membranous positivity in microglia.![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://beta-resources.rndsystems.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251391955.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]
Staining of human lymphoid tissues shows strong membranous positivity in germinal center cells.![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]](https://beta-resources.rndsystems.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251353248.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]")
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP3-43823]
Staining of human liver shows no positivity in hepatocytes as expected.![TMEM119 Antibody (CL8714) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TMEM119 Antibody [NBP3-43823]](https://beta-resources.rndsystems.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251339153.jpg "Immunocytochemistry/ Immunofluorescence: TMEM119 Antibody [NBP3-43823]")
Immunocytochemistry/ Immunofluorescence: TMEM119 Antibody [NBP3-43823]
Staining of SH-SY5Y cells using the Anti-TMEM119 monoclonal antibody).![TMEM119 Antibody (CL8714) - Azide and BSA Free Western Blot: TMEM119 Antibody [NBP3-43823]](https://beta-resources.rndsystems.com/images/products/nbp3-43823_mouse-tmem119-mab-cl8714-azide-and-bsa-free-28220251339127.jpg "Western Blot: TMEM119 Antibody [NBP3-43823]")
Western Blot: TMEM119 Antibody [NBP3-43823]
Analysis in human cell line SH-SY5Y.Applications for TMEM119 Antibody (CL8714) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1 ug/ml
Application Notes
For IHC, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: TMEM119
Long Name
Transmembrane Protein 119
Alternate Names
OBIF
Gene Symbol
TMEM119
Additional TMEM119 Products
Product Documents for TMEM119 Antibody (CL8714) - Azide and BSA Free
Product Specific Notices for TMEM119 Antibody (CL8714) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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