UbcH10/UBE2C Antibody

Novus Biologicals | Catalog # NB100-1084

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all UbcH10/UBE2C Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QETYSKQVTSQEP corresponding to C-Terminus according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1.

Specificity

This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UbcH10/UBE2C Antibody

Western Blot: UbcH10/UBE2C Antibody [NB100-1084]

Western Blot: UbcH10/UBE2C Antibody [NB100-1084]

Western Blot: UbcH10/UBE2C Antibody [NB100-1084] - (2ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084]

Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084]

Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic /Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Flow Cytometry: UbcH10/UBE2C Antibody [NB100-1084]

Flow Cytometry: UbcH10/UBE2C Antibody [NB100-1084]

Flow Cytometry: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084]

Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084]

Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Applications for UbcH10/UBE2C Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1-3 ug/ml
Application Notes
Western blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Primary incubation 1 hour at room temperature.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells.
Flow Cytometry: Flow cytometric analysis of HeLa cells.
Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UbcH10/UBE2C

Ubiquitin-conjugating enzyme E2 C (UBE2C) catalyzes the covalent attachment of ubiquitin to other proteins. Required for the destruction of mitotic cyclins UBE2C carries out the ubiquitin conjugation second step, ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Alternate Names

UBE2C

Entrez Gene IDs

11065 (Human)

Gene Symbol

UBE2C

UniProt

NP_008950. (Human)

Additional UbcH10/UBE2C Products

Product Documents for UbcH10/UBE2C Antibody

Certificate of Analysis

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Product Specific Notices for UbcH10/UBE2C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UbcH10/UBE2C Antibody

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway
Ubiquitination Cascade Pathway Thumbnail