UCH-L1/PGP9.5 Antibody (23GB2135)

Novus Biologicals | Catalog # NBP3-51015

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2135
View all UCH-L1/PGP9.5 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human UCH-L1/PGP9.5 (Uniprot: P09936)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UCH-L1/PGP9.5 Antibody (23GB2135)

UCH-L1/PGP9.5 Antibody (23GB2135)

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (23GB2135)[NBP3-51015] -

Immunocytochemical staining of HAP-1 cells with UCH-L1/PGP9.5 antibody (1:1,000). Nuclei were stained blue with DAPI; UCH-L1/PGP9.5 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
UCH-L1/PGP9.5 Antibody (23GB2135)

Flow Cytometry: UCH-L1/PGP9.5 Antibody (23GB2135)[NBP3-51015] -

Validation of UCH-L1/PGP9.5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with UCH-L1/PGP9.5 antibody (1:2,000) and analyzed using BD flow cytometer.
UCH-L1/PGP9.5 Antibody (23GB2135)

Flow Cytometry: UCH-L1/PGP9.5 Antibody (23GB2135)[NBP3-51015] -

Flow cytometric analysis of UCH-L1/PGP9.5expression in HAP-1 cells using UCH-L1/PGP9.5 antibody (1:2,000). Green, isotype control; red, UCHL1.
UCH-L1/PGP9.5 Antibody (23GB2135)

Western Blot: UCH-L1/PGP9.5 Antibody (23GB2135)[NBP3-51015] -

Western blotting analysis using UCH-L1/PGP9.5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
UCH-L1/PGP9.5 Antibody (23GB2135)

Western Blot: UCH-L1/PGP9.5 Antibody (23GB2135)[NBP3-51015] -

Western blotting analysis using UCH-L1/PGP9.5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit (ubiquitin C-terminal hydrolase L1.

Applications for UCH-L1/PGP9.5 Antibody (23GB2135)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UCH-L1/PGP9.5

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Gene Symbol

UCHL1

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (23GB2135)

Certificate of Analysis

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Product Specific Notices for UCH-L1/PGP9.5 Antibody (23GB2135)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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