VCAM-1/CD106 Antibody (OTI3H10)

Novus Biologicals | Catalog # NBP2-03600

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H10
View all VCAM-1/CD106 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human VCAM1 (NP_001069) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

78.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VCAM-1/CD106 Antibody (OTI3H10)

Western Blot: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Western Blot: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.
Western Blot: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Western Blot: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VCAM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - CD106 / VCAM1 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (OTI3H10) [NBP2-03600]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human Kidney tissue using anti-VCAM1 mouse monoclonal antibody.

Applications for VCAM-1/CD106 Antibody (OTI3H10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VCAM-1/CD106

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq].

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human)

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (OTI3H10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VCAM-1/CD106 Antibody (OTI3H10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VCAM-1/CD106 Antibody (OTI3H10)

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  • Q: I am doing a research product that require investigation of endothelial activation in canine lungs. I see that your VCAM-1 does have reactivity in canines according to your website. Will it work in parafin embedded sections? 

    A:

    Our VCAM-1 antibody with catalogue number NBP2-03600 is covered by our 100% quality guarantee for detection of the canine protein, and for IHC with paraffin-embedded tissue samples. You can read more about our quality guarantee at the following link: quality assurance.

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