Key Product Details

Species Reactivity

Bovine, Equine, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # A18

Format

BSA Free
View all Vitronectin Primary Antibodies »

Product Specifications

Immunogen

Lysed bovine corneal endothelial cells and extracellular matrix

Epitope

Not determined

Specificity

Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). The antibody inhibits integrin-mediated cell adhesion to bovine vitronectin. It can be used to probe vitronectin conformation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse Vitronectin Antibody (A18) - BSA Free (NBP2-23571) is a monoclonal antibody validated for use in IHC and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for Vitronectin Antibody (A18) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
ELISA: NBP2-23571 reacts weakly in ELISA with vitronectin coated directly onto the microtiter plate. IHC: NBP2-23571 is suitable in ELISA and immunostaining of frozen PLP-fixed sections of bovine tissues.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Vitronectin

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Gene Symbol

VTN

Additional Vitronectin Products

Product Documents for Vitronectin Antibody (A18) - BSA Free

Certificate of Analysis

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Product Specific Notices for Vitronectin Antibody (A18) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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Protocols

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FAQs

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