Wnt-5a Antibody (23GB2400)

Novus Biologicals | Catalog # NBP3-51193

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2400
View all Wnt-5a Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Wnt-5a (Uniprot: P41221)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt-5a Antibody (23GB2400)

Wnt-5a Antibody (23GB2400)

Immunocytochemistry/ Immunofluorescence: Wnt-5a Antibody (23GB2400)[NBP3-51193] -

Immunocytochemical staining of HepG2 cells with Wnt-5a antibody (1:1,000). Nuclei were stained blue with DAPI; Wnt-5a was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Wnt-5a Antibody (23GB2400)

Flow Cytometry: Wnt-5a Antibody (23GB2400)[NBP3-51193] -

Flow cytometric analysis of Wnt-5aexpression in HepG2 cells using Wnt family member 5A antibody (1:2,000). Green, isotype control; red, Wnt family member 5A.
Wnt-5a Antibody (23GB2400)

Western Blot: Wnt-5a Antibody (23GB2400)[NBP3-51193] -

Western blotting analysis using Wnt-5a antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Wnt-5a Antibody (23GB2400)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Wnt-5a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 5a

Alternate Names

Wnt5a

Gene Symbol

WNT5A

Additional Wnt-5a Products

Product Documents for Wnt-5a Antibody (23GB2400)

Certificate of Analysis

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Product Specific Notices for Wnt-5a Antibody (23GB2400)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Ligands (Wnts)

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Protocols

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FAQs

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