WT1 Antibody (WT1/3477R)

Novus Biologicals | Catalog # NBP3-07221

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # WT1/3477R
View all WT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa1-100) of human WT1 protein (exact sequence is proprietary) (Uniprot: P19544 )

Localization

Nucleus and cytoplasm. Shuttles between nucleus and cytoplasm.

Marker

Wilm's Tumor & Mesothelial Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08966)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for WT1 Antibody (WT1/3477R)

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221]

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221]

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney. PBS instead of primary antibody. Secondary antibody only negative control. Hematoxylin counterstain.
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221]

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221]

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney sections. Wilm's Tumor 1 labeled using WT1 (WT1/3477R) (1?g/ml). HIER: Tris/EDTA (pH9.0). HRP-Polymer. Hematoxylin counterstain.
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221]

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221]

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney. Secondary antibody only negative control. Hematoxylin counterstain.
WT1 Antibody (WT1/3477R)

WT1 Antibody (WT1/3477R)

Western blot analysis of THP-1 cell lysate using WT1 antibody (WT1/3477R).
WT1 Antibody (WT1/3477R)

WT1 Antibody (WT1/3477R)

Western blot analysis of K562 cell lysate using WT1 antibody (WT1/3477R).
WT1 Antibody (WT1/3477R)

WT1 Antibody (WT1/3477R)

IHC analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma. Strong nuclear staining using WT1 antibody (WT1/3477R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for WT1 Antibody (WT1/3477R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: WT1

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Long Name

Wilms Tumor 1

Alternate Names

GUD, WAGR, WIT-2, WT33

Gene Symbol

WT1

UniProt

P19544 (Human)

Additional WT1 Products

Product Documents for WT1 Antibody (WT1/3477R)

Certificate of Analysis

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Product Specific Notices for WT1 Antibody (WT1/3477R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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