XBP1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77253
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown, Tagged Protein Expression
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human XBP1. The immunogen is located within amino acids 40 - 90 of XBP1.
Specificity
Human XBP1 has two isoforms, including isoform 1 (261aa, 29kD) and isoform 2 (376aa, 40kD). Mouse XBP1 also has two isoforms, including isoform 1 (267aa, 29.6kD) and isoform 2 (371aa, 40kD). And Rat XBP1 has two isoforms as well, including isoform 1 (267aa, 30kD) and isoform 2 (371aa, 40kD). NBP1-77253 can detect human, mouse and rat.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28, 40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for XBP1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] -
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] -
Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - KO Validation in 293T Cells. Loading: 10 u of lysate Antibodies: XBP-1, 4 u/mL and beta-actin, 1u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.![Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]](https://resources.rndsystems.com/images/products/XBP1-Antibody-Western-Blot-NBP1-77253-img0021.jpg "Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]")
Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]
Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p![Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]](https://resources.rndsystems.com/images/products/XBP1-Antibody-Western-Blot-NBP1-77253-img0018.jpg "Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]")
Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]
Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of HepG2 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 1 ug/mL Lane2: 2 ug/mL![Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]](https://resources.rndsystems.com/images/products/XBP1-Antibody-Western-Blot-NBP1-77253-img0019.jpg "Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]")
Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]
Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of A549 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goatanti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1:0.25 ug/mL Lane2: 0.5 ug/mL Lane3: 1 ug/mL![Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]](https://resources.rndsystems.com/images/products/XBP1-Antibody-Western-Blot-NBP1-77253-img0020.jpg "Western Blot: XBP1 AntibodyBSA Free [NBP1-77253]")
Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] -
Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - Validation in Mouse Pancreas Tissue.Loading: 15 u of lysates per lane.Antibodies: XBP-1, (4 u /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] -
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Mouse Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] -
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Liver Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] -
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] -
Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] -
Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Human Liver Tissue.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] -
Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Rat Spleen Tissue.Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.Applications for XBP1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
10-20 ug/ml
Immunohistochemistry
2-5 ug/ml
Immunohistochemistry-Paraffin
2-5 ug/mL
Western Blot
0.25-2 ug/mL
Reviewed Applications
Read 1 review rated 5 using NBP1-77253 in the following applications:
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: XBP1
Long Name
X-box Binding Protein 1
Alternate Names
HTF, TREB5, XBP2
Gene Symbol
XBP1
UniProt
Additional XBP1 Products
Product Documents for XBP1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for XBP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for XBP1 Antibody - BSA Free
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Application: Western BlotSample Tested: mouse liverSpecies: MouseVerified Customer | Posted 09/19/2017ALOX15 knockout attenuated alcohol-induced ER stress
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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