14-3-3 beta Antibody (03) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-06516

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 03

Format

Azide and BSA Free
View all 14-3-3 beta Primary Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 beta (Accession#: NP_003395.1; Met1-Asn246).

Reactivity Notes

Has cross-reactivity in ELISA with:

Cynomolgus YWHAB

No cross-reactivity in ELISA with:

E.coli cell lysate (BL21 cell line)

Specificity

Has cross-reactivity in ELISA with: Cynomolgus YWHABNo cross-reactivity in ELISA with: E.coli cell lysate (BL21 cell line)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 14-3-3 beta Antibody (03) - Azide and BSA Free

Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516]

Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516]

Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516] - Analysis of Human YWHAB expression on Molt-4 cells. The cells were treated, stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for 14-3-3 beta Antibody (03) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 beta

14-3-3 proteins constitute a highly conserved family of homo- and heterodimeric proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs containing phosphorylated serine or threonine residues. There are at least seven distinct 14-3-3 genes in vertebrates: alpha/beta, epsilon, eta, gamma, theta, sigma, and zeta/delta.

Alternate Names

1433 beta, GW128, HS1, YWHAB

Gene Symbol

YWHAB

Additional 14-3-3 beta Products

Product Documents for 14-3-3 beta Antibody (03) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for 14-3-3 beta Antibody (03) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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