15-PGDH/HPGD Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-21588

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%), Canine (93%), Porcine (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 15-PGDH/HPGD Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human 15-PGDH/HPGD. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%), Guinea pig (88%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit 15-PGDH/HPGD Antibody - BSA Free (NBP2-21588) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for 15-PGDH/HPGD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody [NBP2-21588]

Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody [NBP2-21588]

Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NBP2-21588] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP2-21588]

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP2-21588]

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP2-21588] - Human colon carcinoma, using 15-PGDH antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
15-PGDH/HPGD Antibody - BSA Free

Western Blot: 15-PGDH/HPGD Antibody [NBP2-21588] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 15-PGDH antibody (NBP2-21588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for 15-PGDH/HPGD Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 15-PGDH/HPGD

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

15-Hydroxyprostaglandin Dehydrogenase

Alternate Names

15PGDH, HPGD, PGDH1, SDR36C1

Gene Symbol

HPGD

UniProt

P15428 (Human)

Additional 15-PGDH/HPGD Products

Product Documents for 15-PGDH/HPGD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 15-PGDH/HPGD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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