3-OHKYN Antibody (P3UI) [FITC]

Novus Biologicals | Catalog # NB100-597F

Novus Biologicals
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Key Product Details

Species Reactivity

Human, All Species

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # P3UI
View all 3-OHKYN Primary Antibodies »

Product Specifications

Immunogen

3-OHKYN modified KLH

Localization

Cytoplasmic

Specificity

This is specific for 3-hydroxykynureinine (3OH-Kyn) modifications on proteins. It is likely to react with 3OH-Kyn modification on lysine, histidine and cysteine residues on proteins. It does not react with enzymes involved in the degradation of tryptopha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for 3-OHKYN Antibody (P3UI) [FITC]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: 3-OHKYN

3-OHKYN (3-Hydroxykynurenine) is tryptophan's toxic metabolite which is capable of inducing oxidative damage as well as cell death consequently contributing to different neurological and psychiatric disorders. Tryptophan is either transaminated to kynurenate (an endogenous glutamate receptor antagonist), or hydroxylated to 3-OHKYN which is degraded to excitotoxic N-methyl-D-aspartate (NMDA) agonist, quinolinate. Under physiological conditions, kynurenine pathway is activated to form metabolic precursor NAD+; however, under inflammatory, oxidative or neurotoxic attack, there is an increased activation of indoleamine dioxygenase (IDO), kynurenine monooxygenase (KMO) and tryptophan dioxygenase (TDO), yielding the formation of toxic metabolites such as 3-OHKYN and quinolinic acid. Once 3-OHKYN crosses neuronal cell membranes through neutral amino acid transporter (NAT), it can follow two routes: (i) may act as ROS scavenger/antioxidant via direct scavenging or by triggering integral antioxidant responses evoked by transcription signaling pathways, such as the Nrf2/Keap1/ARE axis (ii) 3-OHKYN may undergoes autooxidation yielding highly reactive molecules, such as o-aminoquinone, which in turn causes exacerbated oxidative damage, activation of deadly cascades, and massive cell death.

Alternate Names

3039Hydroxykynurenine, 3-hydroxykynuenine modification

Additional 3-OHKYN Products

Product Documents for 3-OHKYN Antibody (P3UI) [FITC]

Certificate of Analysis

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Product Specific Notices for 3-OHKYN Antibody (P3UI) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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