5-HT3A Antibody (4D5E7) - BSA Free

Novus Biologicals | Catalog # NBP2-61786

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4D5E7

Format

BSA Free
View all 5-HT3A Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human 5-HT3A (AA: extra 24-157) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

55.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-HT3A Antibody (4D5E7) - BSA Free

Western Blot: 5-HT3A Antibody (4D5E7)BSA Free [NBP2-61786]

Western Blot: 5-HT3A Antibody (4D5E7)BSA Free [NBP2-61786]

Western Blot: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)
Immunocytochemistry/ Immunofluorescence: 5-HT3A Antibody (4D5E7) - BSA Free [NBP2-61786]

Immunocytochemistry/ Immunofluorescence: 5-HT3A Antibody (4D5E7) - BSA Free [NBP2-61786]

Immunocytochemistry/Immunofluorescence: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of Hela cells using HTR3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: 5-HT3A Antibody (4D5E7) - BSA Free [NBP2-61786]

Immunohistochemistry-Paraffin: 5-HT3A Antibody (4D5E7) - BSA Free [NBP2-61786]

Immunohistochemistry-Paraffin: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of cervical cancer tissues using HTR3A mouse mAb with DAB staining.
Flow Cytometry: 5-HT3A Antibody (4D5E7) - BSA Free [NBP2-61786]

Flow Cytometry: 5-HT3A Antibody (4D5E7) - BSA Free [NBP2-61786]

Flow Cytometry: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of Hela cells using HTR3A mouse mAb (green) and negative control (red).
Western Blot: 5-HT3A Antibody (4D5E7)BSA Free [NBP2-61786]

Western Blot: 5-HT3A Antibody (4D5E7)BSA Free [NBP2-61786]

Western Blot: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis using HTR3A mAb against HEK293 (1) and HTR3A (AA: extra 24-157)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for 5-HT3A Antibody (4D5E7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT3A

The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.

Long Name

5-Hydroxytryptamine Receptor 3A

Alternate Names

5HT3A, HTR3A

Gene Symbol

HTR3A

Additional 5-HT3A Products

Product Documents for 5-HT3A Antibody (4D5E7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 5-HT3A Antibody (4D5E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for 5-HT3A Antibody (4D5E7) - BSA Free

There are currently no reviews for this product. Be the first to review 5-HT3A Antibody (4D5E7) - BSA Free and earn rewards!

Have you used 5-HT3A Antibody (4D5E7) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies