5-HT7 Antibody (4E6D2)

Novus Biologicals | Catalog # NBP3-15870

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4E6D2 expressed in HEK293
View all 5-HT7 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 5-HT7 (P34969). MMDVNSSGRPDLYGHLRSFLLPEVGRGLPDLSPDGGADPVAGSWAPHLLSEVTASPAPTWDAPPDNASGCGEQINYGRVEKVVIGSILTLITLLTIAGNC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-HT7 Antibody (4E6D2)

Western Blot: 5-HT7 Antibody (4E6D2) [NBP3-15870]

Western Blot: 5-HT7 Antibody (4E6D2) [NBP3-15870]

Western Blot: 5-HT7 Antibody (4E6D2) [NBP3-15870] - Analysis of extracts of various cell lines, using 5-HT7 Rabbit mAb (NBP3-15870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
5-HT7 Antibody (4E6D2)

Immunocytochemistry/ Immunofluorescence: 5-HT7 Antibody (4E6D2) [5-HT7] -

Immunocytochemistry/ Immunofluorescence: 5-HT7 Antibody (4E6D2) [5-HT7] - Confocal imaging of paraffin-embedded Rat brain using 5-HT7 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
5-HT7 Antibody (4E6D2)

Immunocytochemistry/ Immunofluorescence: 5-HT7 Antibody (4E6D2) [5-HT7] -

Immunocytochemistry/ Immunofluorescence: 5-HT7 Antibody (4E6D2) [5-HT7] - Confocal imaging of paraffin-embedded Mouse brain using 5-HT7 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.

Applications for 5-HT7 Antibody (4E6D2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-HT7

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs (

Long Name

5-Hydroxytryptamine Receptor 7

Alternate Names

5-HT-X, 5HT7, HTR7

Gene Symbol

HTR7

Additional 5-HT7 Products

Product Documents for 5-HT7 Antibody (4E6D2)

Certificate of Analysis

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Product Specific Notices for 5-HT7 Antibody (4E6D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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