5-HT7 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56352

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-HT7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).

Reactivity Notes

This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with canine 5-HT7Ra/b, and 81% conserved with porcine 5-HT7R.

Specificity

The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-HT7 Antibody - BSA Free

Western Blot: 5-HT7 Antibody [NB100-56352]

Western Blot: 5-HT7 Antibody [NB100-56352]

Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.
Simple Western: 5-HT7 Antibody [NB100-56352]

Simple Western: 5-HT7 Antibody [NB100-56352]

5-HT7-Antibody-Simple-Western-NB100-56352-img0004.jpg
5-HT7 Antibody

Western Blot: 5-HT7 Antibody [NB100-56352] -

nb100-56352_rabbit-polyclonal-5-ht7-antibody-2092023181152.jpg
5-HT7 Antibody

Simple Western: 5-HT7 Antibody [NB100-56352] -

Simple Western: 5-HT7 Antibody [NB100-56352] - Time-dependent effects of subacute administration of effective dose of escitalopram (5 mg/kg/day) for 3 & 7 days on expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. ** p < 0.01 vs. control by one-way analysis of variance with Tukey’s post-hoc test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for 5-HT7 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 30602786)

Immunocytochemistry/ Immunofluorescence

1:10-1:2000. Use reported in scientific literature (PMID 17940054)

Simple Western

1:50

Western Blot

1-2 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Brain, separated by Size, antibody dilution of 1:50

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT7

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.

Long Name

5-Hydroxytryptamine Receptor 7

Alternate Names

5-HT-X, 5HT7, HTR7

Entrez Gene IDs

3363 (Human); 15566 (Mouse); 65032 (Rat)

Gene Symbol

HTR7

UniProt

P32305 (Rat)

Additional 5-HT7 Products

Product Documents for 5-HT7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for 5-HT7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5-HT7 Antibody - BSA Free

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Protocols

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