5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70166

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F1

Format

Azide and BSA Free
View all 5-Lipoxygenase Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 18-364 of human ALOX5(NP_000689) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

77.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free

Western Blot: 5-Lipoxygenase Antibody (OTI3F1)Azide and BSA Free [NBP2-70166]

Western Blot: 5-Lipoxygenase Antibody (OTI3F1)Azide and BSA Free [NBP2-70166]

Western Blot: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.
Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)
Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)
Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).
Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)
Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166]

Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).

Applications for 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5-Lipoxygenase

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Gene Symbol

ALOX5

Additional 5-Lipoxygenase Products

Product Documents for 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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