5'-Nucleotidase/CD73 Antibody (4G4) - Azide Free

Novus Biologicals | Catalog # NBP1-60135

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G4

Format

Azide Free
View all 5'-Nucleotidase/CD73 Primary Antibodies »

Product Specifications

Immunogen

Inflamed synovial stroma from rheumatoid arthitis patients

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Positive Control: HUVEC and fresh lymphocytes Negative control: COS cells

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (4G4) - Azide Free

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135]

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135]

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135] - Flow cytometric detection of human CD73 on Huvec (mAb 4G4, Cat.# NBP1-60135). Black, red and blue line represents cells only, isotype control and 4G4 (4 ug/ml, respectively).

Applications for 5'-Nucleotidase/CD73 Antibody (4G4) - Azide Free

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:50

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody works in Flow Cytometry, Functional, Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: 5'-Nucleotidase/CD73

The monoclonal antibody 4G4 recognizes both membrane bound and soluble human CD73, also known as ecto-5'-nucleotidase. CD73 is a 70-kDa GPI-anchored cell surface molecule and belongs to the 5'-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5'-nucleotidase activity is an important mediator of the anti-inflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo. In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency. The monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells.

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Entrez Gene IDs

4907 (Human)

Gene Symbol

NT5E

UniProt

P21589

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (4G4) - Azide Free

Certificate of Analysis

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Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (4G4) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5'-Nucleotidase/CD73 Antibody (4G4) - Azide Free

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Protocols

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FAQs for 5'-Nucleotidase/CD73 Antibody (4G4) - Azide Free

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  • Q: We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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