5'-Nucleotidase/CD73 Antibody

Novus Biologicals | Catalog # NBP2-15815

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (93%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all 5'-Nucleotidase/CD73 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human 5'-Nucleotidase/CD73. The exact sequence is proprietary.

Localization

Cell membrane; GPI-anchor

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815]

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815]

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000.
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - CD73 antibody detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: CD73 stained by CD73 antibody diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.
Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815]

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815]

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - J5 xenograft, using CD73 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
5'-Nucleotidase/CD73 Antibody

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] -

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5'-Nucleotidase/CD73 antibody [N1N3] (NBP2-15815) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
5'-Nucleotidase/CD73 Antibody

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] -

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for 5'-Nucleotidase/CD73 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Proximity Ligation Assay

Assay dependent

Western Blot

1:500-1:3000

Neutralization

Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

UniProt

P21589

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 5'-Nucleotidase/CD73 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for 5'-Nucleotidase/CD73 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for 5'-Nucleotidase/CD73 Antibody

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  • Q: We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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