Q: Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.

A: We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

Q: We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?

A: We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.

53BP1 Antibody [PE]

Novus Biologicals | Catalog # NB100-304PE

Submit a review

Citations (1)

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bat, Bovine, Canine, Fish, Goat, Primate

Cited:

Mouse

Predicted:

Chimpanzee (96%), Feline (90%), Orangutan (96%), Porcine (90%), Rabbit (90%), Sheep (90%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), In-situ Hybridization, Knockdown Validated

Cited:

Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Polyclonal Rabbit IgG

View all 53BP1 Primary Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide made to an internal portion of human 53BP1 (between amino acids 350-400) [NP_005648.1].

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32877678). Human and mouse reactivity cited in numerous publications. Primate reactivity reported in scientific literature (PMID: 18389475). Fish reactivity reported in scientific literature (PMID: 25516420). Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). Predicted cross-reactivity based on sequence identity: Chimpanzee (96%), Gorilla (96%), Orangutan (96%), Gibbon (94%), Marmoset (92%), Feline (90%), Porcine (90%), Rabbit (90%), Sheep (90%).

Marker

DNA Double Strand Break Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 53BP1 Antibody [PE]

Flow Cytometry of Jurkat Cells Stained with Conjugated 53BP1 Antibody

1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 ug/mL PE-conjugated 53BP1 Antibody (Catalog #NB100-304PE) in a 150 uL reaction. Isotype control (black), anti-53BP1 (red).