58K Golgi Protein Antibody

Novus Biologicals | Catalog # NB100-1448

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all 58K Golgi Protein Primary Antibodies »

Product Specifications

Immunogen

This 58K Golgi Protein Antibody was developed against a peptide with sequence CLREQGRGKDQPGRL corresponding to internal region according to NP_006648.1, NP_996848.1.

Localization

Cytoplasmic and Golgi Apparatus

Marker

Golgi Apparatus Marker

Specificity

Variants (NP_006648.1; NP_996848.1) encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 58K Golgi Protein Antibody

Western Blot: 58K Golgi Protein Antibody [NB100-1448]

Western Blot: 58K Golgi Protein Antibody [NB100-1448]

Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448]

Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448]

Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: 58K Golgi Protein Antibody [NB100-1448]

Flow Cytometry: 58K Golgi Protein Antibody [NB100-1448]

Flow Cytometry: 58K Golgi Protein Antibody [NB100-1448] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448]

Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448]

Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for 58K Golgi Protein Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
ICC/IF usage is validated and reported in scientific literature (PMID: 16534631).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 58K Golgi Protein

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Alternate Names

formimidoyltransferase cyclodeaminase, formimidoyltransferase-cyclodeaminase, formiminotransferase cyclodeaminase, Formiminotransferase-cyclodeaminase, human formiminotransferase cyclodeaminase, EC 4.3.1.410formiminotransferase-cyclodeaminase, LCHC1

Entrez Gene IDs

10841 (Human)

Gene Symbol

FTCD

UniProt

NP_006648 (Human)

Additional 58K Golgi Protein Products

Product Documents for 58K Golgi Protein Antibody

Certificate of Analysis

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Product Specific Notices for 58K Golgi Protein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 58K Golgi Protein Antibody

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Protocols

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