AATF Antibody (2H6) - Azide and BSA Free

Novus Biologicals | Catalog # H00026574-M09

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2H6

Format

Azide and BSA Free
View all AATF Primary Antibodies »

Product Specifications

Immunogen

AATF (AAH00591, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLT

Specificity

AATF (2H6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for AATF Antibody (2H6) - Azide and BSA Free

Western Blot: AATF Antibody (2H6) [H00026574-M09]

Western Blot: AATF Antibody (2H6) [H00026574-M09]

Western Blot: AATF Antibody (2H6) [H00026574-M09] - Analysis of AATF expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: AATF Antibody (2H6) [H00026574-M09]

Immunocytochemistry/ Immunofluorescence: AATF Antibody (2H6) [H00026574-M09]

Immunocytochemistry/Immunofluorescence: AATF Antibody (2H6) [H00026574-M09] - Analysis of monoclonal antibody to AATF on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: AATF Antibody (2H6) [H00026574-M09] - Detection limit for recombinant GST tagged AATF is 0.03 ng/ml as a capture antibody.

Applications for AATF Antibody (2H6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AATF

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Alternate Names

apoptosis antagonizing transcription factor, Apoptosis-antagonizing transcription factor, CHE-1, CHE1Rb-binding protein Che-1, DEDprotein AATF

Entrez Gene IDs

26574 (Human)

Gene Symbol

AATF

OMIM

608463 (Human)

UniProt

AAH00591 (Human)

Additional AATF Products

Product Documents for AATF Antibody (2H6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AATF Antibody (2H6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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