ABCA12 Antibody

Novus Biologicals | Catalog # NB100-93466

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse

Applications

Validated:

Immunoblotting, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ABCA12 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KDQKSYETADTSSQ corresponding to internal region (near C-Terminus) according to NP_775099.2, NP_056472.2.

Epitope

C-KDQKSYETADTSSQ

Reactivity Notes

Human and Mouse reactivity reported in scientific literature (PMID: 23931754).

Specificity

This antibody is expected to recognize both reported isoforms (NP_775099.2; NP_056472.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for ABCA12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Peptide ELISA

Detection limit 1:1000
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human placenta, skin and testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193 kDa band according to NP_775099.2 and of 257 kDa band according to NP_056472.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IP and ICC/IF usage are reported in scientific literature (PMID: 23931754). Use in immunoblotting reported in scientific literature (PMID: 23931754).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABCA12

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This encoded protein is a member of the ABC1 subfamily, which is the only major ABC subfamily found exclusively in multicellular eukaryotes. Alternative splicing of this gene results in multiple transcript variants.

Alternate Names

ABC12, ATP-binding cassette 12, ATP-binding cassette transporter 12, ATP-binding cassette, sub-family A (ABC1), member 12, DKFZp434G232, EC 3.6.3, EC 3.6.3.25, FLJ41584, ichthyosis congenita II, lamellar ichthyosis B, ICR2B, LI2ATP-binding cassette sub-family A member 12

Entrez Gene IDs

26154 (Human)

Gene Symbol

ABCA12

UniProt

NP_775099.2 (Human)

Additional ABCA12 Products

Product Documents for ABCA12 Antibody

Certificate of Analysis

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Product Specific Notices for ABCA12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ABCA12 Antibody

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Protocols

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