ABCC11 Antibody

Name: ABCC11 Antibody
Brand: Novus Biologicals
SKU: NBP1-20944
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-20944

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-KVVEFDRPEVLRK corresponding to internal region (near C-Terminus) according to NP_115972.2, NP_660187.1.

Epitope

C terminus

Specificity

This antibody is expected to recognize both reported isoforms (NP_115972.2; NP_660187.1). Reported variants represent identical protein (NP_115972.2; NP_149163.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ABCC11 Antibody

Western Blot: ABCC11 Antibody [NBP1-20944]

Western Blot: ABCC11 Antibody [NBP1-20944] - Staining of Human Testes lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: ABCC11 Antibody [NBP1-20944]

Immunocytochemistry/Immunofluorescence: ABCC11 Antibody [NBP1-20944] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: ABCC11 Antibody [NBP1-20944]

Immunohistochemistry-Paraffin: ABCC11 Antibody [NBP1-20944] - Staining of Human Breast tissue. antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: ABCC11 Antibody [NBP1-20944]

Flow Cytometry: ABCC11 Antibody [NBP1-20944] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Applications for ABCC11 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1.5 ug/mL

Application Notes

WB: Approx. 125 kDa band observed in human breast and human testis lysates (calculated MW of 150 kDa band according to NP_660187.1).

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABCC11

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This ABC full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. The product of this gene participates in physiological processes involving bile acids, conjugated steroids, and cyclic nucleotides. In addition, a SNP in this gene is responsible for determination of human earwax type. This gene and family member ABCC12 are determined to be derived by duplication and are both localized to chromosome 16q12.1. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Alternate Names

ATP-binding cassette protein C11, ATP-binding cassette transporter MRP8, ATP-binding cassette transporter sub-family C member 11, ATP-binding cassette, sub-family C (CFTR/MRP), member 11, EWWD, MRP8ATP-binding cassette sub-family C member 11, Multidrug resistance-associated protein 8, multi-resistance protein 8, WW

Entrez Gene IDs

85320 (Human)

Gene Symbol

ABCC11

UniProt

NP_115972.2 (Human)

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Product Documents for ABCC11 Antibody

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Certificate of Analysis

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Product Specific Notices for ABCC11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ABCC11 Antibody

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