ABCG8 Antibody (1B10A5) - BSA Free

Novus Biologicals | Catalog # NBP1-71706

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1B10A5

Format

BSA Free
View all ABCG8 Primary Antibodies »

Product Specifications

Immunogen

Full length mouse ABCG8 [Swiss-Prot# Q9DBM0]

Localization

Membrane; Multi-pass membrane protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for ABCG8 Antibody (1B10A5) - BSA Free

Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706]

Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706]

Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.
Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody (1B10A5) [NBP1-71706]

Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody (1B10A5) [NBP1-71706]

Immunocytochemistry/Immunofluorescence: ABCG8 Antibody (1B10A5) [NBP1-71706] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-ABCG8 Antibody (1B10A5) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706]

Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706]

Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706.

Applications for ABCG8 Antibody (1B10A5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

25 ug

Western Blot

1:1000
Application Notes
This ABCG8 (clone 1B10A5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections.

Reviewed Applications

Read 1 review rated 5 using NBP1-71706 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABCG8

Normal digestion is partially facilitated by the function of ABCG8 and its counterpart ABCG5, which together form a heterodimer which transports cholesterol out of the liver and into bile. Subsequently, cholesterol is eliminated from the bodies of healthy individuals. However, in cases of insulin resistance, this functionality is impaired, and digestion does not result in complete elimination of harmful cholesterol. Unsurprisingly, mutations in ABCG8 can lead to gallstone disease, biliary cancer, hypercholesterolemia and atherosclerosis. In studies related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function and interaction with ABCG5.

Alternate Names

ATP-binding cassette sub-family G member 8, ATP-binding cassette, sub-family G (WHITE), member 8, ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2), GBD4ATP-binding cassette, subfamily G, member 8, MGC142217, sterolin 2, sterolin-2, STSL

Entrez Gene IDs

64240 (Mouse)

Gene Symbol

ABCG8

UniProt

Q9DBM0 (Mouse)

Additional ABCG8 Products

Product Documents for ABCG8 Antibody (1B10A5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ABCG8 Antibody (1B10A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ABCG8 Antibody (1B10A5) - BSA Free

Customer Reviews for ABCG8 Antibody (1B10A5) - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 20210363
    Species: Human
    Verified Customer | Posted 12/24/2014

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Protocols

View specific protocols for ABCG8 Antibody (1B10A5) - BSA Free (NBP1-71706):


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.


Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

*Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ABCG8 Antibody (1B10A5) - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: I have two options for ABCG8 primary antibodies from Novus Biologicals which we can use for fluorescence microscopy. Both of them have not been used/ tested in caco-2 cells (human colon carcinoma cell line) which is our model but for the below: 1) Used for IHC-P in liver, tonsil tissues (species human) (NBP1-83388); 2) Used for IHC-P in mouse intestine (species - mouse) (NBP1-71706SS); Out of the above two which do you and your team think will be more applicable for our purpose.

    A:

    I have spoken to our team and we would recommend using NBP1-83388 and applying for our Innovators Reward Program. Because you are doing ICC staining and we have only tested in tissue. Since it is confirmed to react with human and stain tissue it should probably be a better candidate than testing one which has worked in mouse samples, but not been shown to detect the human form of the protein.

  • Q: Please provide the ratio of the Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3.

    A: The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3 is 0.05M Glycine, 0.5M Tris pH 7.4.

  • Q: What is the concentration of Tris and Glycine in the buffer used for the ABCG8 antibody, NBP1-71706?

    A: The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 is 0.05M Glycine, 0.5M Tris pH 7.4. 

  • Q: I have two options for ABCG8 primary antibodies from Novus Biologicals which we can use for fluorescence microscopy. Both of them have not been used/ tested in caco-2 cells (human colon carcinoma cell line) which is our model but for the below: 1) Used for IHC-P in liver, tonsil tissues (species human) (NBP1-83388); 2) Used for IHC-P in mouse intestine (species - mouse) (NBP1-71706SS); Out of the above two which do you and your team think will be more applicable for our purpose.

    A:

    I have spoken to our team and we would recommend using NBP1-83388 and applying for our Innovators Reward Program. Because you are doing ICC staining and we have only tested in tissue. Since it is confirmed to react with human and stain tissue it should probably be a better candidate than testing one which has worked in mouse samples, but not been shown to detect the human form of the protein.

  • Q: Please provide the ratio of the Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3.

    A: The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3 is 0.05M Glycine, 0.5M Tris pH 7.4.

  • Q: What is the concentration of Tris and Glycine in the buffer used for the ABCG8 antibody, NBP1-71706?

    A: The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 is 0.05M Glycine, 0.5M Tris pH 7.4. 

  • Q: I have two options for ABCG8 primary antibodies from Novus Biologicals which we can use for fluorescence microscopy. Both of them have not been used/ tested in caco-2 cells (human colon carcinoma cell line) which is our model but for the below: 1) Used for IHC-P in liver, tonsil tissues (species human) (NBP1-83388); 2) Used for IHC-P in mouse intestine (species - mouse) (NBP1-71706SS); Out of the above two which do you and your team think will be more applicable for our purpose.

    A:

    I have spoken to our team and we would recommend using NBP1-83388 and applying for our Innovators Reward Program. Because you are doing ICC staining and we have only tested in tissue. Since it is confirmed to react with human and stain tissue it should probably be a better candidate than testing one which has worked in mouse samples, but not been shown to detect the human form of the protein.

  • Q: Please provide the ratio of the Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3.

    A: The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3 is 0.05M Glycine, 0.5M Tris pH 7.4.

  • Q: What is the concentration of Tris and Glycine in the buffer used for the ABCG8 antibody, NBP1-71706?

    A: The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 is 0.05M Glycine, 0.5M Tris pH 7.4. 

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